This practical 2003 handbook provides an extremely comprehensive and highly illustrated guide to micromanipulation techniques in assisted conception in a clinical setting. It includes detailed, illustrated descriptions of all the common micromanipulation systems currently in use in IVF laboratories around the world and clearly explains how to optimise their successful use. The volume covers state-of-the-art techniques including intracytoplasmic sperm injection (ICSI), and procedures such as assisted hatching and the blastomere biopsy (for preimplantation genetic diagnosis PGD). Valuable information on troubleshooting the potential mechanical and technical difficulties that can arise is provided to help all the practitioners of these techniques, including trainee embryologists and consultant obstetricians, and technicians and scientists involved in animal transgenesis and cloning. It will undoubtedly be of immense value to all doctors and scientists working with assisted reproductive technologies.
• Highly illustrated practical guide to the techniques with advice on setting up the equipment, problem solving and troubleshooting • Covers wide range of applications of micromanipulation in ART and IVF • Written by experts with in-depth, hands-on experience of using micromanipulation
Contents
Acknowledgements; Acronyms; Glossary of terms; 1. Micromanipulation in human assisted conception: an overview; 2. Media and other consumables for micromanipulation; 3. Narishige micromanipulation workstation systems; 4. Eppendorf micromanipulation workstation systems; 5. Research Instruments micromanipulation workstation systems; 6. Instrument selection; 7. Preparation of gametes for micromanipulation; 8. Intracytoplasmic sperm injection; 9. Zona manipulation and embryo biopsy; 10. Microtool manufacture; 11. Transgenesis and the generation of knock-out mice; 12. New and advanced techniques; References; Appendix: suppliers and manufacturers of equipment and consumables; Index.
Review
Review of the hardback: 'Its meticulous design, explicit writing style and practical analysis provide a reliable, hands-on manual on assisted conception micromanipulation techniques.' The Obstetrician and Gynaecologist
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