Hostname: page-component-848d4c4894-nr4z6 Total loading time: 0 Render date: 2024-06-08T00:49:10.391Z Has data issue: false hasContentIssue false
  • English
  • Français

In vitro effect of nanosilver on gene expression of superoxide dismutases and nitric oxide synthases in chicken sertoli cells

Published online by Cambridge University Press:  17 September 2014

H. Hassanpour ,
P. Mirshokraei ,
E. Khalili Sadrabad ,
A. Esmailian Dehkordi ,
S. Layeghi ,
A. Afzali  and
A. Mohebbi
H. Hassanpour*
Affiliation:
Research Institute of Animal Embryo Technology, Shahrekord University, P.O. Box 115, Saman Road, Shahrekord, Iran Research Institute of Biotechnology, Shahrekord University, P.O. Box 115, Saman Road, Shahrekord, Iran
P. Mirshokraei
Affiliation:
Research Institute of Animal Embryo Technology, Shahrekord University, P.O. Box 115, Saman Road, Shahrekord, Iran Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Azadi Square, Mashhad, Iran
E. Khalili Sadrabad
Affiliation:
Research Institute of Animal Embryo Technology, Shahrekord University, P.O. Box 115, Saman Road, Shahrekord, Iran
A. Esmailian Dehkordi
Affiliation:
Research Institute of Animal Embryo Technology, Shahrekord University, P.O. Box 115, Saman Road, Shahrekord, Iran
S. Layeghi
Affiliation:
Research Institute of Animal Embryo Technology, Shahrekord University, P.O. Box 115, Saman Road, Shahrekord, Iran
A. Afzali
Affiliation:
Research Institute of Biotechnology, Shahrekord University, P.O. Box 115, Saman Road, Shahrekord, Iran
A. Mohebbi
Affiliation:
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, P.O. Box 115, Saman Road, Shahrekord, Iran
*
†E-mail: Hassanpour-h@vet.sku.ac.ir
Get access

Abstract

To evaluate effects of different concentrations of nanosilver colloid on the cell culture of Sertoli cells, the proportion of lipid peroxidation, antioxidant capacity, nitric oxide (NO) production and genes expression of superoxide dismutases (SOD1 and SOD2) and nitric oxide synthases (eNOS and iNOS) were measured. Sertoli cells were incubated at concentrations of 25, 75 and 125 ppm nanosilver for 48 h. There was progressive lipid peroxidation in treatments according to increasing of nanosilver. Lipid peroxidation, as indicated by malondialdehyde levels, was significantly elevated by the highest concentration of silver colloid (125 ppm), although antioxidant capacity, as measured by ferric ion reduction, was unaffected. Nitrite, as an index of NO production was reduced only in 125 ppm of nanosilver. Expression of SOD1 gene was reduced in nanosilver-treated cells at all concentrations, whereas expression of SOD2 gene was reduced only in cells treated with 125 ppm nanosilver. Expression of iNOS gene was progressively increased with higher concentrations of nanosilver. Expression of eNOS gene was also increased in 125 ppm of nanosilver. In conclusion, toxic effects of nanosilver could be due to high lipid peroxidation and suppression of antioxidant mechanisms via reduced expression of SOD genes and increased expression of NOS genes.

Keywords

nanosilversuperoxide dismutasenitric oxide synthasechicken Sertoli cell
Type
Research Article
Information
animal , Volume 9 , Supplement 2 , February 2015 , pp. 295 - 300
Copyright
© The Animal Consortium 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aiassa, V, Baronetti, JL, Paez, PL, Barnes, AI, Albrecht, C, Pellarin, G, Eraso, AJ and Albesa, I 2011. Increased advanced oxidation of protein products and enhanced total antioxidant capacity in plasma by action of toxins of Escherichia coli STEC. Toxicology In Vitro 25, 426–431.CrossRefGoogle ScholarPubMed
Braydich-Stolle, L, Hussain, S, Schlager, JJ and Hofmann, MC 2005. In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicology Science 88, 412–419.CrossRefGoogle ScholarPubMed
Chairuangkitti, P, Lawanprasert, S, Roytrakul, S, Aueviriyavit, S, Phummiratch, D, Kulthong, K, Chanvorachote, P and Maniratanachote, R 2013. Silver nanoparticles induce toxicity in A549 cells via ROS-dependent and ROS-independent pathways. Toxicology In Vitro 27, 330–338.CrossRefGoogle ScholarPubMed
Chen, X and Schluesener, HJ 2008. Nanosilver: a nanoproduct in medical application. Toxicology Letters 176, 1–12.CrossRefGoogle ScholarPubMed
Coleman, JW 2001. Nitric oxide in immunity and inflammation. International Immunopharmacology 1, 1397–1406.CrossRefGoogle ScholarPubMed
Costa, CS, Ronconi, JVV, Daufenbach, JF, Goncalves, CL, Rezin, GT, Streck, EL and da Silva Paula, MM 2010. In vitro effects of silver nanoparticles on the mitochondrial respiratory chain. Molecular and Cellular Biochemistry 342, 51–56.CrossRefGoogle ScholarPubMed
Farzinpour, A and Karashi, N 2013. The effects of nano-silver on egg quality traits in laying Japanese quail. Applied Nanoscience 3, 59–99.CrossRefGoogle Scholar
Forstermann, U and Sessa, WC 2012. Nitric oxide synthases: regulation and function. European Heart Journal 33, 829–837.CrossRefGoogle Scholar
Foucaud, L, Goulaouic, S, Bennasroune, A, Laval-Gilly, P, Brown, D, Stone, V and Falla, J 2010. Oxidative stress induction by nanoparticles in THP-1 cells with 4-HNE production: stress biomarker or oxidative stress signalling molecule? Toxicology In Vitro 24, 1512–1520.CrossRefGoogle ScholarPubMed
Goudarzi, AK and Hassanpour, H 2007. In vitro production of nitrite by low and high density sperm subpopulations of human, bull and ram. Pakistan Journal of Biological Sciences 10, 2091–2094.Google ScholarPubMed
Grosse, S, Evje, L and Syversen, T 2013. Silver nanoparticle-induced cytotoxicity in rat brain endothelial cell culture. Toxicology In Vitro 27, 305–313.CrossRefGoogle ScholarPubMed
Guibert, E, Briere, S, Pelletier, R, Brillard, JP and Froment, P 2011. Characterization of chicken Sertoli cells in vitro. Poultry Science 90, 1276–1286.CrossRefGoogle ScholarPubMed
Guzik, T, Korbut, R and Adamek-Guzik, T 2003. Nitric oxide and superoxide in inflammation. Journal of Physiology and Pharmacology 54, 469–487.Google ScholarPubMed
Hassanpour, H, Yazdani, A, Soreshjani, KK and Asgharzadeh, S 2009. Evaluation of endothelial and inducible nitric oxide synthase genes expression in the heart of broiler chickens with experimental pulmonary hypertension. British Poultry Science 50, 725–732.CrossRefGoogle ScholarPubMed
Hussain, SM, Hess, KL, Gearhart, JM, Geiss, KT and Schlager, JJ 2005. In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicology In Vitro 19, 975–983.CrossRefGoogle ScholarPubMed
Hussain, SM, Javorina, AK, Schrand, AM, Duhart, HM, Ali, SF and Schlager, JJ 2006. The interaction of manganese nanoparticles with PC-12 cells induces dopamine depletion. Toxicology Science 92, 456–463.CrossRefGoogle ScholarPubMed
Kim, S, Choi, JE, Choi, J, Chung, KH, Park, K, Yi, J and Ryu, DY 2009. Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicology In Vitro 23, 1076–1084.CrossRefGoogle ScholarPubMed
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402–408.CrossRefGoogle ScholarPubMed
Mahmoud, UT 2012. Silver nanoparticles in poultry production. Journal of Advanced Veterinary Research 2, 303–306.Google Scholar
McShan, D, Ray, PC and Yu, H 2014. Molecular toxicity mechanism of nanosilver. Journal of Food & Drug Analysis 22, 116–127.CrossRefGoogle ScholarPubMed
Minaee Zanganeh, B, Roudkenar, M and Kashani, IR 2013. Co-culture of spermatogonial stem cells with sertoli cells in the presence of testosterone and fsh improved differentiation via up-regulation of post meiotic genes. Acta Medica Iranica 51, 1–11.Google ScholarPubMed
Mirshokraei, P, Hassanpour, H, Akhavan Taheri, M, Riyahi, M and Shams-Esfandabadi, N 2011. The in vitro effects of nanosilver colloid on kinematic parameters of ram spermatozoa. Iranian Journal of Veterinary Research 12, 317–323.Google Scholar
Mruk, DD, Silvestrini, B, Mo, M and Cheng, CY 2002. Antioxidant superoxide dismutase – a review: its function, regulation in the testis, and role in male fertility. Contraception 65, 305–311.CrossRefGoogle ScholarPubMed
Mukherjee, SG, O'Claonadh, N, Casey, A and Chambers, G 2012. Comparative in vitro cytotoxicity study of silver nanoparticle on two mammalian cell lines. Toxicology In Vitro 26, 238–251.CrossRefGoogle ScholarPubMed
Park, CJ, Lee, JE, Oh, YS, Shim, S, Nah, WH, Choi, KJ and Gye, MC 2011. Expression of claudin-1 and-11 in immature and mature pheasant (Phasianus colchicus) testes. Theriogenology 75, 445–458.CrossRefGoogle ScholarPubMed
Pineda, L, Sawosz, E, Lauridsen, C, Engberg, RM, Elnif, J, Hotowy, A, Sawosz, F and Chwalibog, A 2012a. Influence of in ovo injection and subsequent provision of silver nanoparticles on growth performance, microbial profile, and immune status of broiler chickens. Open Access Animal Physiology 4, 1–8.Google Scholar
Pineda, L, Chwalibog, A, Sawosz, E, Lauridsen, C, Engberg, R, Elnif, J, Hotowy, A, Sawosz, F, Gao, Y and Ali, A 2012b. Effect of silver nanoparticles on growth performance, metabolism and microbial profile of broiler chickens. Archives of Animal Nutrition 66, 416–429.CrossRefGoogle ScholarPubMed
Rogers, EJ, Hsieh, SF, Organti, N, Schmidt, D and Bello, D 2008. A high throughput in vitro analytical approach to screen for oxidative stress potential exerted by nanomaterials using a biologically relevant matrix: human blood serum. Toxicology In Vitro 22, 1639–1647.CrossRefGoogle ScholarPubMed
Sawosza, E, Bineka, M, Grodzika, M, Zieliñskaa, M, Sysaa, P, Szmidt, M, Niemiec, T and Chwalibog, A 2007. Infunce of hydrocolloidal silver nanoparticles on gastrointestinal microflora and morphology of enterocytes of quails. Archives of Animal Nutrition 61, 444–451.CrossRefGoogle Scholar
Teodoro, JS, Simoµes, AM, Duarte, FV, Rolo, AP, Murdoch, RC, Hussain, SM and Palmeira, CM 2011. Assessment of the toxicity of silver nanoparticles in vitro: a mitochondrial perspective. Toxicology In Vitro 25, 664–670.CrossRefGoogle ScholarPubMed
Teshfam, M, Nikbakht Brujeni, G and Hassanpour, H 2006. Evaluation of endothelial and inducible nitric oxide synthase mRNA expression in the lung of broiler chickens with developmental pulmonary hypertension due to cold stress. British Poultry Science 47, 223–229.CrossRefGoogle ScholarPubMed
Troy, CM, Derossi, D, Prochiantz, A, Greene, LA and Shelanski, ML 1996. Downregulation of Cu/Zn superoxide dismutase leads to cell death via the nitric oxide-peroxynitrite pathway. Journal of Neuroscience 16, 253–261.CrossRefGoogle ScholarPubMed
Valko, M, Leibfritz, D, Moncol, J, Cronin, MTD, Mazur, M and Telser, J 2007. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry and Cell Biology 39, 44–84.CrossRefGoogle ScholarPubMed
Wijnhoven, SWP, WJGM, Peijnenburg, Herberts, CA, Hagens, WI, Oomen, AG, Heugens, EHW, Roszek, B, Bisschops, J, Gosens, I and Van De Meent, D 2009. Nano-silver – a review of available data and knowledge gaps in human and environmental risk assessment. Nanotoxicology 3, 109–138.CrossRefGoogle Scholar
Yagi, K 1998. Simple assay for the level of total lipid peroxides in serum or plasma. Methods in Molecular Biology 108, 101–106.Google ScholarPubMed
Yamakura, F and Kawasaki, H 2010. Post-translational modifications of superoxide dismutase. Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics 1804, 318–325.CrossRefGoogle ScholarPubMed
Zanette, C, Pelin, M, Crosera, M, Adami, G, Bovenzi, M, Larese, FF and Florio, C 2011. Silver nanoparticles exert a long-lasting antiproliferative effect on human keratinocyte HaCaT cell line. Toxicology In Vitro 25, 1053–1060.CrossRefGoogle ScholarPubMed
Supplementary material: File

Hassanpour Supplementary Material

Table S1

Download Hassanpour Supplementary Material(File)
File 34.8 KB