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Modifications of the G6G timed-AI protocol improved pregnancy per AI and reduced pregnancy loss in lactating dairy cows

Published online by Cambridge University Press:  15 March 2017

F. Heidari ,
E. Dirandeh ,
Z. Ansari Pirsaraei  and
M. G. Colazo
F. Heidari
Affiliation:
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, PO Box 578, Sari, Mazandaran, Iran
E. Dirandeh*
Affiliation:
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, PO Box 578, Sari, Mazandaran, Iran
Z. Ansari Pirsaraei
Affiliation:
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, PO Box 578, Sari, Mazandaran, Iran
M. G. Colazo
Affiliation:
Livestock Research Section, Alberta Agriculture and Forestry, Edmonton, AB, Canada, T6H 5T6
*
†E-mail: Dirandeh@gmail.com
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Abstract

In dairy cows, subjected to a G6G protocol, objectives were to determine effects of (1) extending the interval from prostaglandin F2α (PGF2α) to gonadotropin-releasing hormone (GnRH) during presynchronization; and (2) adding a second PGF2α treatment before artificial insemination (AI), on ovarian response, plasma progesterone (P4) concentrations and pregnancy per AI (P/AI). In a 2×2 factorial design, lactating cows were randomly assigned to one of four timed AI (TAI) protocols: (1) G6G (n=149), one injection of PGF2α, GnRH 2 days later and a 7-day Ovsynch (GnRH, 7 days, PGF2α, 56 h, GnRH, 16 h, TAI) was initiated 6 days later; (2) G6GP (n=144), an additional PGF2α treatment (24 h after the first) during Ovsynch of the G6G protocol; (3) MG6G, one injection of PGF2α, GnRH 4 days later before initiation of the G6G protocol; and (4) MG6GP, an additional PGF2α treatment (24 h after the first) during Ovsynch of the MG6G protocol. Blood samples were collected (subset of 200 cows) at first GnRH and PGF2α of the Ovsynch, and at TAI to measure P4. Ultrasound examinations were performed in a subset of 406 cows to evaluate ovarian response at various times of Ovsynch, and in all cattle to determine pregnancy status at 32 and 60 days after TAI. Extending the interval by 2 days between PGF2α and GnRH during presynchronization increased (P<0.01) ovulatory response to first GnRH of Ovsynch, circulating P4 during Ovsynch, and P/AI at 32 and 60 days after TAI. Adding a second PGF2α treatment before AI increased the proportion of cows with luteal regression (P=0.04), improved P/AI at 60 days after TAI (P=0.05), and reduced pregnancy loss between 30 and 60 days after TAI (P=0.04). In summary, extending the interval from PGF2α to GnRH during presynchronization increased response to first GnRH of Ovsynch and P4 concentrations during Ovsynch, whereas adding a second PGF2α treatment before AI enhanced luteal regression. Both modifications of the G6G protocol improved fertility in lactating dairy cows.

Keywords

timed AIpregnancypregnancy lossPGF2αdairy cow
Type
Research Article
Information
animal , Volume 11 , Issue 11 , November 2017 , pp. 2002 - 2009
Copyright
© The Animal Consortium and Her Majesty the Queen in Right of Canada, represented by the Minister of Agriculture and Agri-Food Canada and the Minister of Health Canada 2017 

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