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Effect of supplementing a fibre basal diet with different nitrogen forms on ruminal fermentation and microbial growth in an in vitro semi-continuous culture system (RUSITEC)

Published online by Cambridge University Press:  09 March 2007

M. D. Carro  and
E. L. Miller
M. D. Carro*
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071 León, Spain
E. L. Miller
Affiliation:
Department of Clinical Veterinary Medicine, University of Cambridge, Nutrition Laboratory, 307 Huntingdon Road, Cambridge CB3 0JQ, UK
*
*Corresponding author: Dr Dolores Carro, fax +34 987 291311, email DP1MCT@UNILEON.ES
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Abstract

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Incubation trials were carried out with the rumen simulation technique (RUSITEC) to study the effects of four forms of N on the growth of ruminal micro-organisms and the fermentation variables when an all-fibre basal diet was incubated. The basal diet consisted of 10 g neutral-detergent fibre (NDF) from grass hay plus 2 g NDF from sugarbeet pulp. N forms were isolated soyabean protein, soyabean peptides, amino acids blended to profile soyabean protein and NH3 as NH4Cl. Half of the daily N supply was infused as NH4Cl and the other half was infused as each of the four treatments described. Non-NH3 N (NAN) forms increased NDF (P = 0·006), acid-detergent fibre (P = 0·003) and cellulose (P = 0·015) disappearance after 48 h incubation, CO2 (P < 0·001), CH4 (P = 0·002) and total volatile fatty acids production (P < 0·001), as well as the molar percentages of isobutyrate, isovalerate and valerate, which reflected the fermentation of amino acid C skeletons. NAN treatments also increased microbial N flow (P < 0·001) compared with NH3, with peptides and protein supporting more (P = 0·036) than amino acids. The proportion of microbial N derived from NH3 decreased successively (P < 0·05) with NH3 > amino acids > peptides > protein treatments, indicating preferential uptake of peptides without passage through the NH3 pool. Microbial efficiency (g microbial N/kg organic matter apparent disappearance) was greater (P = 0·002) for the NAN forms than for the NH3 treatment, with peptides and protein treatments supporting higher (P = 0·009) values than amino acid treatment. These results indicate that N forms other than NH3 are required for optimal fibre digestion and microbial growth.

Keywords

NitrogenMicrobial protein synthesisRUSITEC
Type
Research Article
Information
British Journal of Nutrition , Volume 82 , Issue 2 , August 1999 , pp. 149 - 157
Copyright
Copyright © The Nutrition Society 1999

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