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The symbiotic complex of Dendroctonus simplex: implications in the beetle attack and its life cycle

Published online by Cambridge University Press:  26 February 2019

A.-A. Durand ,
P. Constant ,
E. Déziel  and
C. Guertin Open the ORCID record for C. Guertin [Opens in a new window]
A.-A. Durand
Affiliation:
INRS-Institut Armand Frappier, 531 Boul. Des Prairies, Laval, Québec, Canada, H7V 1B7
P. Constant
Affiliation:
INRS-Institut Armand Frappier, 531 Boul. Des Prairies, Laval, Québec, Canada, H7V 1B7
E. Déziel
Affiliation:
INRS-Institut Armand Frappier, 531 Boul. Des Prairies, Laval, Québec, Canada, H7V 1B7
C. Guertin*
Affiliation:
INRS-Institut Armand Frappier, 531 Boul. Des Prairies, Laval, Québec, Canada, H7V 1B7
*
*Author for correspondence Phone: (450) 687-5010 ext. 4117 Fax: (450) 686-5511 E-mail: Claude.Guertin@iaf.inrs.ca
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Abstract

The eastern larch beetle (Dendroctonus simplex Le Conte) is recognized as a serious destructive forest pest in the upper part of North America. Under epidemic conditions, this beetle can attack healthy trees, causing severe damages to larch stands. Dendroctonus species are considered as holobionts, as they engage in multipartite interactions with microorganisms, such as bacteria, filamentous fungi, and yeasts, which are implicated in physiological processes of the insect, such as nutrition. They also play a key role in the beetle's attack, as they are responsible for the detoxification of the subcortical environment and weaken the tree's defense mechanisms. The eastern larch beetle is associated with bacteria and fungi, but their implication in the success of the beetle remains unknown. Here, we investigated the bacterial and fungal microbiota of this beetle pest throughout its ontogeny (pioneer adults, larvae and pupae) by high-throughput sequencing. A successional microbial assemblage was identified throughout the beetle developmental stages, reflecting the beetle's requirements. These results indicate that a symbiotic association between the eastern larch beetle and some of these microorganisms takes place and that this D. simplex symbiotic complex is helping the insect to colonize its host tree and survive the conditions encountered.

Keywords

Dendroctonusdevelopmental stagesmicrobiotamicrobial communitieshigh throughput sequencing
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 109 , Issue 6 , December 2019 , pp. 723 - 732
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Copyright
Copyright © Cambridge University Press 2019 

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