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Myelin Basic Protein: A Component of Circulating Immune Complexes in Multiple Sclerosis

Published online by Cambridge University Press:  18 September 2015

Mrinal K. Dasgupta ,
Ingrid Catz ,
Kenneth G. Warren ,
Thomas A. McPherson ,
John B. Dossetor  and
Patrick R. Carnegie
Mrinal K. Dasgupta*
Affiliation:
Department of Medicine (Nephrology and Clinical Immunology), University of Alberta, Edmonton, Alberta
Ingrid Catz
Affiliation:
Cross Cancer Institute, Edmonton, Alberta
Kenneth G. Warren
Affiliation:
Department of Medicine (Neurology), University of Alberta, Edmonton, Alberta
Thomas A. McPherson
Affiliation:
Cross Cancer Institute, Edmonton, Alberta
John B. Dossetor
Affiliation:
Department of Medicine (Nephrology and Clinical Immunology), University of Alberta, Edmonton, Alberta
Patrick R. Carnegie
Affiliation:
Cross Cancer Institute, Edmonton, Alberta
*
Room 1046. University of Alberta Hospital, Edmonton, Alberta, Canada, T6G 2B7.
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Summary:

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Myelin basic protein (MBP) is an antigenic component of circulating immune complexes (CIC) in patients with multiple sclerosis (MS). Immune complexes were isolated from the sera by adsorption to Raji cells and then acid eluted. Final identification of MBP from Raji eluates was done by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by MBP radioimmunoassay (RIA) of gel eluates and by an immunoblot technique.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 10 , Issue 4 , November 1983 , pp. 239 - 243
Copyright
Copyright © Canadian Neurological Sciences Federation 1983

References

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