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Bone morphogenetic protein receptor IB as a candidate gene for prolificacy in Small Tail Han and Hu ewes

Published online by Cambridge University Press:  13 June 2008

Yan Ya-Dong ,
Chu Ming-Xing ,
Zeng Yong-Qing ,
Fang Li ,
Ye Su-Cheng ,
Wang Li-Min ,
Guo Qing-Kun ,
Han Dai-Qin ,
Zhang Zhao-Xin  and
Wang Xi-Jun
...Show all authors
Yan Ya-Dong
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China
Chu Ming-Xing*
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Zeng Yong-Qing
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China
Fang Li
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Ye Su-Cheng
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Wang Li-Min
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
Guo Qing-Kun
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
Han Dai-Qin
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
Zhang Zhao-Xin
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
Wang Xi-Jun
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
Zhang Zhao-Xin
Affiliation:
Animal Husbandry Bureau of Jiaxiang County, Shandong Province, Jiaxiang 272400, China
*
*Corresponding author. Email: mxchu@263.net
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Abstract

The bone morphogenetic protein receptor IB (BMPR-IB) gene, which controls the fecundity of Booroola Merino ewes, was studied as a candidate gene for the prolificacy of Small Tail Han and Hu ewes. A single nucleotide polymorphism of the BMPR-IB gene was detected in both high (Small Tail Han and Hu) and low (Suffolk and Dorset) fecundity sheep breeds by polymerase chain reaction–single-strand conformation polymorphism (PCR-SSCP) analysis. The results indicated the presence of the same mutation (A746G) of the BMPR-IB gene in both Small Tail Han and Hu ewes and in Booroola Merino ewes, but not in both Suffolk and Dorset ewes. In Small Tail Han ewes, frequencies of BB, B+ and ++ genotypes were 0.524, 0.383 and 0.093, respectively. In Hu ewes, these frequencies were 0.882, 0.118 and 0.000. The BMPR-IB genotype distributions were significantly different (P<0.001) among high- and low-fecundity sheep breeds. Small Tail Han ewes with genotype BB had 0.92 (P<0.01) and 1.02 (P<0.01) lambs more than those with genotype ++ in the first and second parity, respectively. These results demonstrated that the BMPR-IB gene is a major gene affecting the prolificacy in both Small Tail Han and Hu ewes, and could be used as a molecular genetic marker to select the litter size in sheep.

Keywords

sheepprolificacybone morphogenetic protein receptor IB genePCR-SSCP
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 2 , August 2005 , pp. 125 - 130
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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