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Construction of antagonistic, insecticidal, and endophytic strains of Bacillus spp. by protoplast fusion

Published online by Cambridge University Press:  13 June 2008

Yan Nian-Long ,
Qiu Si-Xin ,
He Hong ,
Guan Xiong  and
Hu Fang-Ping
Yan Nian-Long
Affiliation:
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Qiu Si-Xin
Affiliation:
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China
He Hong
Affiliation:
Agricultural College, Zhanjiang Ocean University, Zhanjiang 324088, China
Guan Xiong
Affiliation:
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Hu Fang-Ping*
Affiliation:
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
*Corresponding author. Email: Huf@fjau.edu.cn
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Abstract

A multifunctional-engineering strain was constructed by fusing the insecticidal bacterium (Bacillus thuringiensis) with vip3A gene and the endophytic bacterium (B. subtilis) protoplasts. Results of polymerase chain reaction (PCR) showed that the insecticidal vip3A gene of B. thuringiensis was present in the fusant. The fusant's antagonism to Fusarium oxysporum f. sp. cucumerinum, the adjusted mortality to Plutella xylostella and the endophytic characters were detected. The result showed that the antagonism of the fusant was against the F. oxysporum f. sp. cucumerinum; the adjusted mortality to P. xylostella reached 59% and it had insecticidal characteristics.

Keywords

vip3A geneendophyticprotoplast fusion
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 2 , August 2005 , pp. 131 - 135
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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