Hostname: page-component-848d4c4894-p2v8j Total loading time: 0.001 Render date: 2024-06-04T06:18:18.824Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular tagging of a novel stripe rust resistance gene, YrSp, derived from a spelt wheat derivative

Published online by Cambridge University Press:  12 February 2007

Wei Yan-Ling ,
Ni Zhong-Fu ,
Liu Qing ,
Xie Chao-Jie ,
Yang Tso-Min  and
Sun Qi-Xin
Wei Yan-Ling
Affiliation:
Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100094, China
Ni Zhong-Fu
Affiliation:
Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100094, China
Liu Qing
Affiliation:
Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100094, China
Xie Chao-Jie
Affiliation:
Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100094, China
Yang Tso-Min
Affiliation:
Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100094, China
Sun Qi-Xin*
Affiliation:
Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mali: QXSUN62@public.bta.net.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

Ninety-nine individual plants of a segregating F2 population of a wheat single cross Wen 6×CP90.0.2.4.1 were used in this study to find out whether CP90.0.2.4.1, a derivative of Triticum spelta var. album, carries a novel stripe rust resistance gene other than Yr5. Using molecular marker technology, it was found that only one out of 150 SSR primers could produce amplified polymorphism, and a marker, Xgwm155-147bp, located on chromosome 3A, was found to be linked to the stripe rust resistance gene identified with a genetic distance of 40.5 cM, indicating that the latter was also located on chromosome 3A. Since the only Yr gene previously reported to originate from T. spelta var. album is Yr5, located on 2BL, the only logical inference is that there might be other Yr gene (genes) in T. spelta, and the Yr gene identified in the present study might be a novel one. It was temporarily designated YrSp.

Keywords

spelt wheatstripe rustresistance genemolecular markerchromosome location
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 1 , April 2004 , pp. 45 - 48
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Chen, XM, Line, RF and Jones, SS (1995) Chromosomal location of genes for stripe rust resistance in spring wheat cultivars Compare, Fielder, Lee, and Lemhi and interactions of aneuploid wheat with races of Puccinia striiformis. Phytopathology 85: 375–381.CrossRefGoogle Scholar
Gardiner, JM, Coe, EH, Melia-Hancock, S et al. , (1993) Development of a core RFLP map in maize using an immortalized F2 population. Genetics 134: 917–930.CrossRefGoogle ScholarPubMed
Ma, JX (1999) Identification, location and molecular mapping of a novel wheat stripe rust resistance gene. PhD thesis, Chinese Academy of Agricultural Sciences (in Chinese).Google Scholar
Macer, RCF (1966) The formal and monosomic genetic analysis of stripe rust (Puccinia striiformis) resistance in wheat. In: Mackey, J (editor) Proceedings of the 2nd International Wheat Genetics Symposium, Lund, Sweden 1963. Hereditas Supplement. 2: 127–142.Google Scholar
McIntosh, RA, Hart, GE, Devos, KM et al. , (1998) Proceedings of the 9th International Wheat Genetics Symposium, Vol. 5: Catalogue of Gene Symbols for Wheat. Canada: University of Saskatchewan, University Extension Press. 140 ppGoogle Scholar
Michelmore, RW, Paran, L and Kesseli, RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of the USA 88: 9828–9832.CrossRefGoogle ScholarPubMed
Peng, JH, Fahima, T, Röder, MS et al. , (1999) Microsatellite tagging of the stripe-rust resistance gene YrH52 derived from wild emmer wheat, Triticum dicoccoides, and suggestive negative crossover interference on chromosome 1B. Theoretical and Applied Genetics 98: 862–872.CrossRefGoogle Scholar
Robert, O, Abelard, C and Dedryver, F (1999) Identification of molecular markers for the detection of the stripe rust resistance gene Yr17 in wheat. Molecular Breeding 5: 167–175.CrossRefGoogle Scholar
Röder, MS, Korzun, V, Wendehake, K et al. , (1998) A microsatellite map of wheat. Genetics 149: 2007–2023.CrossRefGoogle ScholarPubMed
Sun, GL, Fahima, T, Korol, AB et al. , (1997) Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides. Theoretical and Applied Genetics 95: 622–628.CrossRefGoogle Scholar
Wang, FL Wu, LR, Xu SC et al. , (1996) Systematic investigations on the breakdown of resistance in wheat cultivars of Mianyang derivatives to stripe rust. Acta Phytopathologica Sinica 26: 105–109. (in Chinese with English abstract)Google Scholar
Wang, LF (1999) Genetic analysis and molecular marker of wheat stripe rust and resistance gene. MS thesis, Chinese Academy of Agricultural Sciences (in Chinese).Google Scholar
Wei, YL (1999) Microsatellite marker for two stripe rust resistance gene in wheat Yr5 introgressed from spelt wheat (Triticum spelta) to common wheat (Triticum aestivum). PhD thesis, Chinese Agricultural University (in Chinese).Google Scholar
Yang, TM, Tang, BR, Shen, KQ et al. , (1993) Identification and utilization of diversified sources of resistance to the rusts and mildew in China. In: Li, ZS and Xin, ZY (editors) Proceeding of the Eighth International Wheat Genetics Symposium Beijing: China Agricultural Scientech Press, 913–916.Google Scholar