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Population genetic diversity of Phytophthora infestans from China as revealed by SSRs and RAPDs

Published online by Cambridge University Press:  30 October 2009

Li Ben-Jin ,
Lv Xin ,
Chen Qing-He ,
Lan Cheng-Zhong ,
Zhao Jian ,
Qiu Rong-Zhou  and
Weng Qi-Yong
Li Ben-Jin
Affiliation:
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Lv Xin
Affiliation:
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China Central Laboratory, Fujian Academy of Agricultural Sciences/Key Laboratory of Test in Agricultural Field by Precision Type Instrument, Fuzhou 350003, China
Chen Qing-He
Affiliation:
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Lan Cheng-Zhong
Affiliation:
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Zhao Jian
Affiliation:
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Qiu Rong-Zhou
Affiliation:
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Weng Qi-Yong*
Affiliation:
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China Central Laboratory, Fujian Academy of Agricultural Sciences/Key Laboratory of Test in Agricultural Field by Precision Type Instrument, Fuzhou 350003, China
*
*Corresponding author. E-mail: wengqy@faas.cn
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Abstract

Simple sequence repeats (SSR) and random amplification of polymorphic DNA (RAPD) molecular markers were used to assess the genetic diversity of 80 isolates of Phytophthora infestans in potato (Solanum tuberosum) from Fujian, Heilongjiang, Hebei and Inner Mongolia Provinces in China. Polymorphism was identified by 13 SSR primers and 14 RAPD primers in the isolates of P. infestans in potato. A total of 76 bands were amplified by SSRs, with the percentage of polymorphic bands (PPB) being 78.9% and the similarity coefficient ranging between 0.00 and 0.42. A total of 189 bands were amplified by RAPDs, with the percentage of polymorphic bands being 95.2% and the similarity coefficient ranging between 0.04 and 0.66. Analysis of genetic diversity showed that there exists higher genetic variation in the Fujian population in comparison to the populations of Heilongjiang, Hebei and Inner Mongolia. Nei's genetic identity analysis indicates that the genetic similarity between populations of Heilongjiang and Inner Mongolia is the highest and that between Fujian and Hebei is the lowest. A cluster analysis revealed that isolates from Fujian, in the south of China, are distantly related to those from Heilongjiang, Hebei and Inner Mongolia in the north, and the Fujian population is distributed among more groups than the other three, exhibiting a higher genetic diversity.

Keywords

potatoPhytophthora infestansSSRRAPDgenetic diversity
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 2 , August 2009 , pp. 161 - 170
Copyright
Copyright © China Agricultural University 2009

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