Hostname: page-component-848d4c4894-5nwft Total loading time: 0 Render date: 2024-06-02T00:13:57.760Z Has data issue: false hasContentIssue false
  • English
  • Français

Maximal lactate steady state for aerobic evaluation of swimming mice

Published online by Cambridge University Press:  22 October 2009

Claudio Alexandre Gobatto ,
Fúlvia de Barros Manchado-Gobatto ,
Ligia Giuzio Carneiro ,
Gustavo Gomes de Araujo  and
Ivan Gustavo Masselli dos Reis
Claudio Alexandre Gobatto*
Affiliation:
Department of Physical Education, Laboratory of Sport Applied Physiology, Sao Paulo State University – UNESP, Av. 24A, 1515, Bela Vista, 13506-900Rio Claro, SP, Brazil
Fúlvia de Barros Manchado-Gobatto
Affiliation:
Department of Physical Education, Laboratory of Sport Applied Physiology, Sao Paulo State University – UNESP, Av. 24A, 1515, Bela Vista, 13506-900Rio Claro, SP, Brazil
Ligia Giuzio Carneiro
Affiliation:
Department of Physical Education, Laboratory of Sport Applied Physiology, Sao Paulo State University – UNESP, Av. 24A, 1515, Bela Vista, 13506-900Rio Claro, SP, Brazil
Gustavo Gomes de Araujo
Affiliation:
Department of Physical Education, Laboratory of Sport Applied Physiology, Sao Paulo State University – UNESP, Av. 24A, 1515, Bela Vista, 13506-900Rio Claro, SP, Brazil
Ivan Gustavo Masselli dos Reis
Affiliation:
Department of Physical Education, Laboratory of Sport Applied Physiology, Sao Paulo State University – UNESP, Av. 24A, 1515, Bela Vista, 13506-900Rio Claro, SP, Brazil
*
*Corresponding author: cgobatto@pq.cnpq.br
Get access

Abstract

The maximal lactate steady state (MLSS) has been considered the gold standard method to determine aerobic/anaerobic metabolism transition during continuous exercise executed by human beings and rats. Therefore, the aim of the present study was to determine MLSS for aerobic evaluation in swimming mice. Twenty-five adult male mice (90-day-old animals) were adapted to the deep aquatic environment at the temperature of 31 ± 1°C. The mice were submitted to five continuous exercise loads of 3, 4, 5, 6 and 7% of the body weight (bw) tied to the back, executed with 25-min duration and 48-h intervals between them. Blood samples were collected from the tail during swimming exercise (rest, 5, 10, 15, 20 and 25 min) for blood lactate concentration (LAC) determinations. The individual MLSS was considered as the highest intensity in which the increase on the LAC was equal to or below 1 mmol l− 1 from the 10th to the 25th minute of exercise. The results showed that 36% of the swimming mice presented MLSS at 4% bw, 20% at 3% bw and 6% bw, 16% at 5% bw and 8% at 7% bw. The LAC at the MLSS was 5.78 ± 0.29 mmol l− 1 (4.40–6.67 mmol l− 1). These results indicate that the MLSS of mice swimming with additional weight for the final 15 of 25 min of exercise could be determined.

Keywords

blood lactateaerobic exercise intensityswimming miceanaerobic threshold
Type
Short Communication
Information
Comparative Exercise Physiology , Volume 6 , Issue 3 , August 2009 , pp. 99 - 103
Copyright
Copyright © Cambridge University Press 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Wasserman, K and McIlroy, MB (1964). Detecting the threshold of anaerobic metabolism in cardiac patients during exercise. American Journal of Cardiology 14: 844–852.CrossRefGoogle ScholarPubMed
2 Kindermann, W, Simon, G and Keul, J (1979). The significance of the aerobic–anaerobic transition for the determination of work load intensities during endurance training. European Journal of Applied Physiology 42: 25–34.CrossRefGoogle ScholarPubMed
3 Mader, A, Liesen, H, Heck, H, Philippi, H, Rost, R, Schürich, P, et al. (1976). Zur Beurteilung der sportartspezifischen Ausdauerleistungsfähigkeit im Labor. Sportarzt und Sportmedizin 27: 80–88 (Teil I) and 109–112 (Teil II).Google Scholar
4 Tegtbur, U, Busse, MW and Braumann, KM (1993). Estimation of an individual equilibrium between lactate production and catabolism during exercise. Medicine and Science in Sports and Exercise 25: 620–627.CrossRefGoogle ScholarPubMed
5 Chassain, A (1986). Méthode d'appréciation objetive de la tolérance de l'organisme á l'effort: application á la mensure des puissances de la frequence cardiaque et de la lactatémie. Science & Sports 1: 41–48.CrossRefGoogle Scholar
6 Monod, H and Scherer, J (1965). The work capacity of a synergic muscular group. Ergonomics 8: 329–338.CrossRefGoogle Scholar
7 Billat, VL, Siverent, P, Py, G, Korallsztein, JP and Mercier, J (2003). The concept of maximal lactate steady state: a bridge between biochemistry, physiology and sport science. Sports Medicine 33: 407–426.CrossRefGoogle Scholar
8 Beneke, R (2003). Methodological aspects of maximal lactate steady state – implications for performance testing. European Journal of Applied Physiology 89: 95–99.CrossRefGoogle ScholarPubMed
9 Mader, A and Heck, H (1986). A theory of metabolic origin of the anaerobic threshold. International Journal of Sports Medicine 7: S45–S65.CrossRefGoogle ScholarPubMed
10 Harnish, CR, Swensen, TC and Pate, RP (2001). Methods for estimating the maximal lactate steady state in trained cyclists. Medicine and Science in Sports and Exercise 33: 1052–1055.CrossRefGoogle Scholar
11 Gobatto, CA, Kokubun, E, Sibuya, CY and Mello, MAR (1991). Efeitos da desnutrição protéico-calórica e do treinamento físico na produção de ácido lático em ratos machos adultos após teste de cargas progressivas. Ciência e Cultura 43: 725–726.Google Scholar
12 Gobatto, CA, Mello, MAR, Sibuya, CY, Azevedo, JRM, Santos, LA and Kokubun, E (2001). Maximal lactate steady state in rats submitted to swimming exercise. Comparative Biochemistry and Physiology – Part A: Molecular & Integrative Physiology 130: 21–27.CrossRefGoogle Scholar
13 Marangon, L, Gobatto, CA, Mello, MAR and Kokubun, E (2002). Utilization of an hyperbolic model for the determination of the critical load in swimming rats. Medicine and Science in Sports and Exercise 34: S149.CrossRefGoogle Scholar
14 Voltarelli, FA, Gobatto, CA and Mello, MAR (2002). Determination of anaerobic threshold in rats using the lactate minimum test. Brazilian Journal of Medical Biology Research 35: 1–6.CrossRefGoogle ScholarPubMed
15 Manchado, FB, Gobatto, CA, Contarteze, RVL, Papoti, M and Mello, MAR (2005). Maximal lactate steady state in running rats. Journal of Exercise Physiology 8: 29–35.Google Scholar
16 Manchado, FB, Gobatto, CA, Contarteze, RVL, Papoti, M and Mello, MAR (2006 a). The maximal lactate steady state is ergometer-dependent in an experimental model using rats. Revista Brasileira de Medicina do Esporte 12: 259–262.CrossRefGoogle Scholar
17 Gobatto, CA, Manchado, FB, Voltarelli, FA, Contarteze, RVL and Mello, MAR (2005). Non-invasive critical load determination in swimming rats: effects of muscle glycogen depletion. Medicine and Science in Sports and Exercise 37: S331.Google Scholar
18 Manchado, FB, Gobatto, CA, Voltarelli, FA and Mello, MAR (2006 b). Non-exhaustive test for aerobic capacity determination in swimming rats. Applied Physiology, Nutrition and Metabolism 31: 731–736.CrossRefGoogle ScholarPubMed
19 Araujo, GG, Papoti, M, Manchado, FB, Mello, MAR and Gobatto, CA (2007). Protocols for hyperlactatemia induction in the lactate minimum test adapted to swimming rats. Comparative Biochemistry and Physiology – Part A: Molecular & Integrative Physiology 148: 888–892.CrossRefGoogle ScholarPubMed
20 Contarteze, RVL, Manchado, FB, Gobatto, CA and Mello, MAR (2008). Stress biomarkers in rats submitted to swimming and treadmill running exercises. Comparative Biochemistry and Physiology – Part A: Molecular & Integrative Physiology 151: 415–422.CrossRefGoogle ScholarPubMed
21 Billat, VL, Mouisel, E, Roblot, N and Melki, J (2005). Inter- and intra-strain variation in mouse critical running speed. Journal of Applied Physiology 98: 1258–1263.CrossRefGoogle Scholar
22 Ferreira, JCB, Rolim, NPL, Bartholomeu, JB, Gobatto, CA, Kokubun, E and Brum, PC (2007). Maximal lactate steady state in running mice: effect of exercise training. Clinical and Experimental Pharmacology and Physiology 34: 760–765.CrossRefGoogle Scholar
23 Heck, H, Mader, A, Hess, G, Mücke, S, Müller, R and Hollmann, W (1985). Justification of the 4-mmol.l− 1 lactate threshold. International Journal of Sports Medicine 6: 117–130.CrossRefGoogle Scholar
24 Beneke, R (1995). Anaerobic threshold, individual anaerobic threshold, and maximal lactate steady state in rowing. Medicine and Science in Sports and Exercise 27: 863–867.CrossRefGoogle Scholar
25 Pilis, W, Zarzeczny, R, Langfort, J, Kaciuba-Uscilko, H, Nazar, K and Wojtyna, J (1993). Comparative Biochemistry and Physiology – Part A: Physiology 106: 285–289.CrossRefGoogle Scholar