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‘1001’ Campylobacters: cultural characteristics of intestinal campylobacters from man and animals

Published online by Cambridge University Press:  19 October 2009

M. B. Skirrow  and
J. Benjamin
M. B. Skirrow
Affiliation:
Microbiology Department, Worcester Royal Infirmary, Worcester WR1 3AS
J. Benjamin
Affiliation:
Microbiology Department, Worcester Royal Infirmary, Worcester WR1 3AS
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Summary

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The cultural characteristics of 1220 Campylobacter strains from a variety of sources are described. Forty-two were identified as Campylobacter fetus ssp. fetus (Véron & Chatelain, 1973), 1120 as members of the C. jejuni/C. coli group, and 58 did not conform to any known description. Sixteen of the latter strains had the basic characteristics of C. fetus but were atypical in certain other respects. The other 42 strains had the thermophilic characteristics of the jejuni/coli group, but were resistant to nalidixie acid and had other features in common; it is possible that they represent a new species. They were isolated from 19% of locally caught wild seagulls but only occasionally from other animals and man.

Growth at 25 °C clearly distinguished strains of C. fetus from those of the jejuni/coli and the nalidixie acid-resistant thermophilic (NARTC) groups. Maximum growth temperature was less reliable for this purpose, and 43 °C was found to be better than the traditional 42 °C. By arranging the results of three tests (tolerance to 2,3,5-triphenyltetrazolium chloride, growth at 30·5 and 45·5 °C) serially in the form of a schema comprising nine categories, the jejuni/coli strains fell into two main groups resembling the Institute Pasteur C. jejuni and C. coli type strains, but these groups could not be clearly defined owing to the existence of strains with intermediate characteristics.

Most of the strains from cattle resembled C. jejuni, whereas those from pigs resembled C. coli; poultry strains occupied a more intermediate position. Strains from man and other animals were of mixed types, but most human strains resembled C. jejuni rather than C. coli. The type distribution pattern that most nearly matched that of human indigenous strains was given by a half-and-half mixture of strains from cattle and poultry.

Type
Research Article
Information
Journal of Hygiene , Volume 85 , Issue 3 , December 1980 , pp. 427 - 442
Copyright
Copyright © Cambridge University Press 1980

References

REFERENCES

Approved Lists of Bacterial Names (1980). International Journal of Systematic Bacteriology 30, 225–420.Google Scholar
Butzler, J. P., Dekeyser, P., Detrain, M. & Dehaen, F. (1973). Related vibrio in stools. Journal of Pediatrics 82, 493–5.CrossRefGoogle ScholarPubMed
Cook, G. T. (1950). A plato test for nitrate reduction. Journal of Clinical Pathology 3, 359.CrossRefGoogle Scholar
Doyle, L. P. (1948). The etiology of swine dysentery. American Journal of Veterinary Research 9, 50–1.Google Scholar
George, H. A., Hoffman, P. S., Smibert, R. M. & Krieg, N. R. (1978). Improved media for growth and aerotolerance of Campylobacter fetus. Journal of Clinical Microbiology 8, 36–41.CrossRefGoogle ScholarPubMed
Gordon, R. E. (1978). A species definition. International Journal of Systematic Bacteriology 28, 605–7.CrossRefGoogle Scholar
Hoffman, P. S., George, H. A., Krieg, N. R. & Smibert, R. M. (1979 a). Studies of the microaerophilic nature of Campylbacter fetus subsp. jejuni. II. Role of exogenous superoxide anions and hydrogen peroxide. Canadian Journal of Microbiology 25, 8–16.CrossRefGoogle ScholarPubMed
Hoffman, P. S., Krieg, N. R. & Smibert, R. M. (1979 b). Studies of the microaorophilic naturo of Campylobacter fetus subsp. jejuni. I. Physiological aspects of enhanced aerotolerance. Canadian Journal of Microbiology 25, 1–7.CrossRefGoogle Scholar
Hugh, R. & Leifson, E. (1953). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. Journal of Bacteriology 66, 24–6.CrossRefGoogle ScholarPubMed
Jones, F. S., Orcutt, M. & Little, R. B. (1931). Vibrios (Vibrio jejuni, n.sp.) associated with intestinal disorders of cows and calves. Journal of Experimental Medicine 53, 853–64.CrossRefGoogle ScholarPubMed
King, E. O. (1957). Human infections with Vibrio fetus and a closely related vibrio. Journal of Infectious Diseases 101, 119–28.CrossRefGoogle Scholar
Mayfield, C.I. & Inniss, W. E. (1977). A rapid, simple method for staining bacterial flagella. Canadian Journal of Microbiology 23, 1311–13.CrossRefGoogle ScholarPubMed
Skirrow, M. B. (1977). Campylobactor enteritis – a ‘new’ disease. British Medical Journal ii, 9–11.CrossRefGoogle Scholar
Smibert, R. M. (1974). In Bergey's Manual of Determinative Bacteriology. 8th ed., pp. 207–11. Baltimore: Williams & Wilkins.Google Scholar
Thomas, K., Chan, K. N. & Ribeiro, C. D. (1980). Campylobacter jejuni/coli meningitis in a neonate. British Medical Journal i, 1301.CrossRefGoogle Scholar
Tribe, G. W., Mackenzie, P. S. & Fleming, M. P. (1979). Incidence of Thermophilic Campylobacter species in newly imported simian primates with enteritis (Letter). Veterinary Record 105, 333.CrossRefGoogle Scholar
Vanhoof, R., Vanderlinden, M. P., Dierickx, R., Lauwers, S., Yourassowsky, E. & Butzler, J. P. (1978). Susceptibility of Campylobacter fetus subsp. jejuni to twenty-nine antimicrobial agents. Antimicrobial Agents and Chemotherapy 14, 553–6.CrossRefGoogle ScholarPubMed
Véron, M. & Chatelain, R. (1973). Taxonomic study of The gonus Campylobacter Sebald and Véron and designation of The neotype strain for the type species Campylobacter fetus (Smith and Taylor) Sebald and Véron. International Journal of Systematic Bacteriology 23, 122–34.CrossRefGoogle Scholar