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Detection and quantification of IgM, IgA, IgG1 and IgG2 antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay

Published online by Cambridge University Press:  25 March 2010

E. M. E. Abu Elzein  and
J. R. Crowther
E. M. E. Abu Elzein
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey GU24 0NF
J. R. Crowther
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey GU24 0NF
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Summary

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A simple solid-phase enzyme immunoassay is described for the detection of antibody classes showing activity against foot-and-mouth disease (FMD) virus in bovine sera. The assay achieves a preliminary separation of the specific class of antibody from other serum proteins through immuno-adsorption to class-specific immunoglobulin-coated wells of micro-titre plates. The specific antibody is reacted with FMD virus, which is then detected by an enzyme-labelled anti virus IgG.

Type
Research Article
Information
Journal of Hygiene , Volume 86 , Issue 1 , February 1981 , pp. 79 - 85
Copyright
Copyright © Cambridge University Press 1981

References

REFERENCES

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