Epidemiological characterization of methicillin-resistant Staphylococcus aureus isolated in the North West of England by protein A (spa) and coagulase (coa) gene polymorphisms

J. WALKER; R. BORROW; V. EDWARDS-JONES; B. A. OPPENHEIM; A. J. FOX

doi:10.1017/S0950268898001538

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In a comparative study, isolates of methicillin-resistant Staphylococcus aureus (MRSA) with known pulsed-field gel electrophoresis (PFGE) and bacteriophage type were analysed by polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) for additional discriminatory subtyping information. PFGE was previously performed using standardized, commercially available kits and pre-programmed software. Isolates were examined for coagulase (coa) and protein A (spa) gene polymorphisms following PCR amplification of the coa hypervariable and spa repeat regions. Coa gene RFLPs produced a total of 38 distinct combined patterns after digestion with HaeIII and AluI and identified the predominant epidemic (EMRSA) types 15 and 16. A unique HaeIII restriction site was identified by RFLP and sequence analysis in the coa gene for EMRSA 15 but not EMRSA 16. The spa gene PCR yielded a total of 14 different profiles ranging from 3–18 repeats with the 2 predominant EMRSA types falling into 2 distinct groups. PCR detection of coa and spa polymorphisms offer a rapid preliminary strain identification and discriminatory subtyping information for surveillance of MRSA.

Crossref Citations

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Tondo, E C Guimarães, MC M Henriques, J AP and Ayub, M AZ 2000. Assessing and analysing contamination of a dairy products processing plant byStaphylococcus aureususing antibiotic resistance and PFGE. Canadian Journal of Microbiology, Vol. 46, Issue. 12, p. 1108.

VAN BELKUM, ALEX 2000. Molecular Epidemiology of Methicillin-ResistantStaphylococcus aureusStrains: State of Affairs and Tomorrow's Possibilities. Microbial Drug Resistance, Vol. 6, Issue. 3, p. 173.

Wichelhaus, Thomas A. Hunfeld, Klaus-Peter Böddinghaus, Boris Kraiczy, Peter Schàfer, Volker and Brade, Volker 2001. Rapid Molecular Typing of Methicillin-ResistantStaphylococcus aureusby PCR-RFLP. Infection Control & Hospital Epidemiology, Vol. 22, Issue. 5, p. 294.

Nahvi, Massoumeh D. Fitzgibbon, Joseph E. John, Joseph F. and Dubin, Donald T. 2001. Sequence Analysis ofdruRegions from Methicillin-ResistantStaphylococcus aureusand Coagulase-Negative Staphylococcal Isolates. Microbial Drug Resistance, Vol. 7, Issue. 1, p. 1.

Montesinos, I. Salido, E. Delgado, T. Cuervo, M. and Sierra, A. 2002. Epidemiologic Genotyping of Methicillin-Resistant Staphylococcus aureus by Pulsed-Field Gel Electrophoresis at a University Hospital and Comparison with Antibiotyping and Protein A and Coagulase Gene Polymorphisms . Journal of Clinical Microbiology, Vol. 40, Issue. 6, p. 2119.

Walker, J. Fox, A.J. Edwards-Jones, V. and Gordon, D.B. 2002. Intact cell mass spectrometry (ICMS) used to type methicillin-resistant Staphylococcus aureus: media effects and inter-laboratory reproducibility. Journal of Microbiological Methods, Vol. 48, Issue. 2-3, p. 117.

Štěpán, J. Pantůček, R. and Doškař, J. 2004. Molecular diagnostics of clinically important staphylococci. Folia Microbiologica, Vol. 49, Issue. 4, p. 353.

Enright, Mark C. and Witte, Wolfgang 2004. Staphylococcus Aureus. p. 31.

Edwards-Jones, V. Buck, R. Shawcross, S.G. Dawson, M.M. and Dunn, K. 2004. The effect of essential oils on methicillin-resistant Staphylococcus aureus using a dressing model. Burns, Vol. 30, Issue. 8, p. 772.

Palazzo, I. C. V. Araujo, M. L. C. and Darini, A. L. C. 2005. First Report of Vancomycin-Resistant Staphylococci Isolated from Healthy Carriers in Brazil. Journal of Clinical Microbiology, Vol. 43, Issue. 1, p. 179.

Vimercati, C. Cremonesi, P. Castiglioni, B. Boettcher, P. Luzzana, M. Ruffo, G. and Moroni, P. 2005. Analysis of Genetic Polymorphisms in Staphylococcus aureus Strains Isolated from Bovine Milk. Veterinary Research Communications, Vol. 29, Issue. S2, p. 257.

Jury, F. Al-Mahrous, M. Apostolou, M. Sandiford, S. Fox, A. Ollier, W. and Upton, M. 2006. Rapid cost-effective subtyping of meticillin-resistant Staphylococcus aureus by denaturing HPLC. Journal of Medical Microbiology , Vol. 55, Issue. 8, p. 1053.

Potel, C. Álvarez, M. Álvarez, P. Otero, I. and Fluiters, E. 2007. Evolution, antimicrobial susceptibility and assignment to international clones of methicillin-resistant Staphylococcus aureus isolated over a 9-year period in two Spanish hospitals. Clinical Microbiology and Infection, Vol. 13, Issue. 7, p. 728.

Çiftci, Alper Onuk, E. Emek Findik, Arzu Yildirim, Tuba and Sogut, Mehtap Unlu 2009. Molecular Typing of Staphylococcus Aureus Strains from Ovine Mastitis by Pulsed-Field Gel Electrophoresis and Polymerase Chain Reaction Based on Coagulase and Protein A Gene Polymorphisms. Journal of Veterinary Diagnostic Investigation, Vol. 21, Issue. 6, p. 849.

Saei, Habib Dastmalchi and Ahmadi, Malahat 2012. Discrimination of Staphylococcus aureus isolates on the basis of gene coding protein A using PCR-restriction enzyme analysis. Comparative Clinical Pathology, Vol. 21, Issue. 5, p. 645.

Lasch, Peter Fleige, Carola Stämmler, Maren Layer, Franziska Nübel, Ulrich Witte, Wolfgang and Werner, Guido 2014. Insufficient discriminatory power of MALDI-TOF mass spectrometry for typing of Enterococcus faecium and Staphylococcus aureus isolates. Journal of Microbiological Methods, Vol. 100, Issue. , p. 58.

Khichar, Vikas Kataria, Anil Kumar and Sharma, Ramavtar 2014. Characterization of Staphylococcus aureus of cattle mastitis origin for two virulence-associated genes (coa and spa). Comparative Clinical Pathology, Vol. 23, Issue. 3, p. 603.

Eed, EM Ghonaim, MM Hussein, YM Al-Shehri, SS and Khalifa, AS 2016. Molecular characterisation of Panton–Valentine leucocidin-producing methicillin-resistant Staphylococcus aureus clones isolated from the main hospitals in Taif, KSA. Indian Journal of Medical Microbiology, Vol. 34, Issue. 4, p. 476.

de Almeida, Camila C. Pizauro, Lucas J. L. Soltes, Glenn A. Slavic, Durda de Ávila, Fernando A. Pizauro, João M. and MacInnes, Janet I. 2018. Some coagulase negative Staphylococcus spp. isolated from buffalo can be misidentified as Staphylococcus aureus by phenotypic and Sa442 PCR methods. BMC Research Notes, Vol. 11, Issue. 1,

Kareem, S.M. Aljubori, S.S. and Ali, M.R. 2020. Novel determination of spa gene diversity and its molecular typing among Staphylococcus aureus Iraqi isolates obtained from different clinical samples. New Microbes and New Infections, Vol. 34, Issue. , p. 100653.

Article contents

Epidemiological characterization of methicillin-resistant Staphylococcus aureus isolated in the North West of England by protein A (spa) and coagulase (coa) gene polymorphisms

Abstract

Save article to Kindle

Save article to Dropbox

Save article to Google Drive

Reply to: Submit a response

Your details

You have entered the maximum number of contributors

Conflicting interests