Hostname: page-component-848d4c4894-4hhp2 Total loading time: 0 Render date: 2024-06-02T10:59:53.647Z Has data issue: false hasContentIssue false
  • English
  • Français

Interactions of TRIC agents with macrophages: effects on lysosomal enzymes of the cell

Published online by Cambridge University Press:  15 May 2009

Janice Taverne ,
W. A. Blyth  and
R. C. Ballard
Janice Taverne
Affiliation:
Medical Research Council Trachoma Unit, The Lister Institute of Preventive Medicine, Chelsea Bridge Road, London SWlW 8RH
W. A. Blyth
Affiliation:
Medical Research Council Trachoma Unit, The Lister Institute of Preventive Medicine, Chelsea Bridge Road, London SWlW 8RH
R. C. Ballard
Affiliation:
Medical Research Council Trachoma Unit, The Lister Institute of Preventive Medicine, Chelsea Bridge Road, London SWlW 8RH
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two changes were observed in the acid phosphatase of macrophages that had ingested infective TRIC organisms: the proportion of extralysosomal enzyme rose, while the total amount in the cells fell. Both effects were directly related to the number of organisms ingested and increased with time. When macrophages were inoculated with more than 50 organisms per cell, changes were obvious within a few hours; with 2–10 organisms per cell changes were detectable only after 18 hr. or more. Enzyme appeared in the culture medium as the amount in the cells decreased. Ingestion of organisms killed by heat or treated with antibody did not induce such changes. In infected BHK-21 cells, no changes in acid phosphatase were detected at any stage of the developmental cycle of the organism.

Type
Research Article
Information
Journal of Hygiene , Volume 72 , Issue 2 , April 1974 , pp. 297 - 309
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Blyth, W. A., Taverne, J. & Garrett, A. J. (1971). Trachoma inclusions separated from cells. In Trachoma and Related Disorders. Proceedings of a Symposium Held in Boston, Mass. August 1970 (ed. Nichols, R. L.), pp. 79–87. Amsterdam: Excerpta Medica.Google Scholar
Blyth, W. A. & Taverne, J. (1974). Neutralization of TRIC organisms by antibody: enhancement by antisera prepared against immunoglobulins. Journal of Hygiene 72, 129.CrossRefGoogle ScholarPubMed
Cohn, Z. A. & Wiener, E. (1963). The particulate hydrolases of macrophages. II. Biochemical and morphological response to particle ingestion. Journal of Experimental Medicine 118, 1009–20.CrossRefGoogle ScholarPubMed
Jones, B. R. (1961). Trachoma and allied infections. Transactions of the Ophthalmological Society of the United Kingdom 81, 367–78.Google ScholarPubMed
Kordova, N., Wilt, J. C. & Sadiq, M. (1971). Lysosomes in L cells infected with Chlamydia psittaci 6BC strain. Canadian Journal of Microbiology 17, 955–9.CrossRefGoogle Scholar
Lawn, A. M., Blyth, W. A. & Taverne, J. (1973). Interactions of TRIC organisms with macrophages and BHK-21 cells observed by electron microscopy. Journal of Hygiene 71, 1–14.CrossRefGoogle ScholarPubMed
Murray, E. S., Bell, S. D. Jr, Hanna, A. T., Nichols, R. L. & Snyder, J. C. (1960). Studies on Trachoma. 1. Isolation and identification of strains of elementary bodies from Saudi Arabia and Egypt. American Journal of Tropical Medicine 9, 116–24.Google ScholarPubMed
Reeve, P. & Taverne, J. (1963). Observations on the growth of trachoma and inclusion blennorrhoea viruses in embryonate eggs. Journal of Hygiene 61, 67–75.CrossRefGoogle Scholar
Schoenholtz, W. K. (1962). Experimental Studies on Lethal and Immune Mechanisms in Massive Bedsonia Infections. PhD Dissertation, University of California, Berkeley, California.Google Scholar
Taverne, J. & Blyth, W. A. (1971). Interactions between trachoma organisms and macrophages. In Trachoma and Belated Disorders. Proceedings of a Symposium Held in Boston, Mass. August 1970 (ed. Nichols, R. L.), pp. 88–107. Amsterdam: Excerpta Medica.Google Scholar
Weissmann, G. & Thomas, L. (1962). Studies on lysosomes. 1. The effects of endotoxin, endotoxin tolerance and cortisone on the release of acid hydrolases from a granular fraction of rabbit liver. Journal of Experimental Medicine 116, 433–50.CrossRefGoogle Scholar
Wolff, D. A. & Bubel, H. C. (1964). The disposition of lysosomal enzymes as related to specific viral cytopathic effects. Virology 24, 502–5.CrossRefGoogle ScholarPubMed