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Isolation of verocytotoxin-producing Escherichia coli from milk filters in south-western Ontario

Published online by Cambridge University Press:  15 May 2009

R. C. Clarke ,
S. A. McEwen ,
V. P. Gannon ,
H. Lior  and
C. L. Gyles
R. C. Clarke
Affiliation:
Health of Animals Laboratory, Agriculture Canada, 110 Stone Road West, Guelph, Ontario, Canada, NW 3W4
S. A. McEwen
Affiliation:
Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, NIG 2W1
V. P. Gannon
Affiliation:
Animal Diseases Research Institute, Agriculture Canada, Lethbridge, Alberta, Canada, T1J 3Z4
H. Lior
Affiliation:
National Enteric Reference Centre, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada, K1A 0L2
C. L. Gyles
Affiliation:
Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, NIG 2W1
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A total of 1012 milk filters collected from 498 dairy farms in south-western Ontario during three study periods (December 1985–March 1986) were tested for the presence of verocytotoxin-producing Escherichia coli (VTEC). VTEC were detected and isolated using a Vero cell assay. Supernatants from 20 of the milk filter cultures had verocytotoxic activity and 7 VTEC strains were isolated from these positive samples. The prevalence of VTEC in the samples in each of the three study periods were 0·44, 0·65 and 0·99% respectively. All seven VTEC strains isolated were sensitive to commonly used antimicrobial agents. The serotypes of these strains were O 26.H11, O43.H2, O 153.H25, O ?. H8, O ?. H19, O ?.non-motile, and Orough. H19. Two of these serotypes (O 153. H25 and O 26.H11) have previously been associated with disease in humans.

Type
Research Article
Information
Epidemiology & Infection , Volume 102 , Issue 2 , April 1989 , pp. 253 - 260
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

Bopp, C. A., Greene, K. D., Downes, F. P., Sowers, E. G., Wells, G. J. & Wachsmuth, I. K. (1987). Unusual verotoxin-producing Escherichia coli associated with hemorrhagic colitis. Journal of Clinical Microbiology 25, 489–497.Google Scholar
Borczyk, A. A., Karmali, M. A., Lior, H. & Duncan, L. M. C. (1987). Bovine reservior for verotoxin-producing Escherichia coli O 157. H7. Lancet 1, 98.Google Scholar
Cahoon, F. E. & Thompson, J. S. (1987). Frequency of Escherichia coli O 157. H7 isolation from stool specimens. Canadian Journal of Microbiology 33, 914–915.CrossRefGoogle Scholar
Clarke, R. C., Mcewen, S., Harnett, N., Lior, H. & Gyles, C. (1988). The prevalence of verotoxin-producing Escherichia coli in bovines at slaughter. Abstract P-48. 88th Annual Meeting of the American Society for Microbiology. Miami Beach, 8–1305.Google Scholar
Doyle, M. P. & Schoeni, J. L. (1984). Survival and growth characteristics of Escherichia coli associated with hemorrhagic colitis. Applied and Environmental Microbiology 48, 855–856.CrossRefGoogle ScholarPubMed
Doyle, M. P. & Schoeni, J. L. (1987). Isolation of Escherichia coli O 157. H7 from retail fresh meats and poultry. Applied and Environmental Microbiology 53, 2394–2396.Google Scholar
Duncan, L., Mai, V., Carter, A., Carlson, J., Borczyk, A. & Karmali, M. (1987). Outbreak of gastrointestinal disease — Ontario. Canadian Diseases Weekly Report 13, 5–8.Google Scholar
Gannon, V. P., Gyles, C. L. & Friendship, R. W. (1988). Characteristics of verotoxigenic Escherichia coli from pigs. Canadian Journal of Veterinary Research 52, 331–337.Google ScholarPubMed
Harris, A. A., Kaplan, L. J., Goodman, L. J., Doyle, M., Laundau, W., Segreti, J., Mayer, K. & Levin, S. (1985). Results of a screening method used in a 12-month stool survey for Escherichia coli o 157. H7. Journal of Infectious Diseases 152, 775–777.CrossRefGoogle Scholar
Ish-Shalom, N., Arbus, G. S., Karmali, M. A. & Petric, M. (1987). Clinical and prognostic features of Verotoxin-producing E. coli- associated hemolytic uremic syndrome. Abstract HUS- 11. International Symposium and Workshop on Verocytotoxin-producing Escherichia coli infections. Toronto.Google Scholar
Johnson, W. M., Lior, H. & Bezanson, G. S. (1983). Cytotoxic Escherichia coli O 157. H7 associated with hemorrhagic colitis in Canada. Lancet 1, 76.CrossRefGoogle Scholar
Karmali, M. A. (1989). Verocytotoxin-producing Escherichia coli infections. Reviews of Clinical Microbiology. In press.CrossRefGoogle Scholar
Karmali, M. A., Petric, M., Lim, C., Fleming, P. C., Arbus, G. S. & Lior, H. (1985). The association between hemolytic uremic syndrome and infection by Verotoxin-producing Escherichia coli. Journal of Infections Diseases 151, 775–782.Google Scholar
Karmali, M. A., Steele, B. T., Petric, M. & Lim, C. (1983). Sporadic cases of hemolytic uremic syndrome associated with fecal cytotoxin and cytotoxin-producing Escherichia coli. Lancet 1, 619–620.CrossRefGoogle ScholarPubMed
Konowalchuk, J., Piers, J. I. & Stavric, S. (1977). Vero response to a cytotoxin of Escherichia coli. Infection and Immunity 18, 775–779.CrossRefGoogle ScholarPubMed
Krishnan, C., Fitzgerald, V. A., Dakin, S. J. & Behme, R. J. (1987). Laboratory investigation of an outbreak of hemorrhagic colitis caused by Escherichia coli O 157. H7. Journal of Clinical Microbiology 25, 1043–1047.CrossRefGoogle Scholar
Levine, M. M., Xu, J.-G., Kaper, J. B., Lior, H., Prado, V., Tall, B., Nataro, J., Karch, H. & Wachsmuth, I. K. (1987). A DNA probe to identify enterohemorrhagic Escherichia coli of O 157. H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome. Journal of Infectious Diseases 156, 175–182.Google Scholar
Mai, V., Carter, A., Duncan, L., Carlson, J. & Borczyk, A. (1987). Kindergarten outbreak of Escherichia coli O 157. H7 infection associated with a farm visit. International Symposium and Workshop on Verocytotoxin-producing Escherichia coli infections. Toronto, 07 12– 15.Google Scholar
Mainil, J., Duchesnes, C., Whipp, S., Marques, L., O'Brien, A., Asey, T. & Moon, H. (1987). Shiga-like toxin production and attaching effacing activity of Escherichia coli associated with calf diarrhea. American Journal of Veterinary Research 48, 743–748.Google Scholar
March, S. B. & Ratnam, S. (1986). Sorbitol—MacConkey medium for detection of Escherichia coli O 157. H7 associated with hemorrhagic colitis. Journal of Clinical Microbiology 23, 869–872.CrossRefGoogle Scholar
Martin, M. L., Shipman, L. D., Wells, J. G., Potter, M. E., Hedberg, K., Wachsmuth, I. K., Tauxe, R. V., Davis, J. P., Arnoldi, J. & Tilleli, J. (1986). Isolation of Escherichia coli O 157. H7 from dairy cattle associated with two cases of haemolytic-uraemic syndrome Lancet 2, 1043.CrossRefGoogle Scholar
Mcewen, S. A., Martin, S. W., Clarke, R. C., Tamblyn, S. E. & McDermott, J. J. (1988).The prevalence, incidence, geographical distribution, antimicrobial sensitivity patterns and plasmid profiles of milk filter Salmonella isolates from Ontario dairy farms. Canadian Journal of Veterinary Research 52, 18–22.Google ScholarPubMed
Mohammed, A., Peiris, J. S. M. & Wijenwanta, E. A. (1986). Serotypes of verocytotoxigenic Escherichia coli from cattle and buffalo calf diarrhea. FEMS microbiology Letters 35, 261–265.CrossRefGoogle Scholar
Mohammed, A., Peiris, J. S. M., Wijewanta, E. A., Mahalingham, S. & Gunasekara, G. (1985). Role of Verocytotoxigenic Escherichia coli in cattle and buffalo calf diarrhea. FEMS Microbiology Letters 26, 281–283.CrossRefGoogle Scholar
Moxley, R. A. & Francis, D. H. (1986). Natural and experimental infection with an attaching and effacing strain of Escherichia coli in calves. Infection and Immunity 53, 339–346.CrossRefGoogle ScholarPubMed
Neill, M. A., Agosti, J. & Rosen, H. (1985). Hemorrhagic colitis due to Escherichia coli 0157: H7 preceding adult hemolytic—uremic syndrome. Archives of Internal Medicine 145, 2215–2217.CrossRefGoogle Scholar
Orskov, F., Orskov, I. & Villar, J. A. (1987). Cattle as a reservoir of Verocytoxin-producing Escherichia coli O 157. H7. Lancet 2, 276.Google Scholar
Pal, C. H., Ahmed, N., Lior, H., Johnson, W.M., Sims, H. V. & Woods, D. E. (1988). Epidemiology of sporadic diarrhea due to verocytotoxin-producing Escherichia coli: a two- year prospective study. Journal of Infectious Diseases 157, 1054–1057.Google Scholar
Pal, C. H., Gordon, R., Sims, H. V. & Bryan, L. E. (1984). Sporadic cases of hemorrhagic colitis associated with Escherichia coli O 157. H7. Annals of Internal Medicine 101, 738–742.Google Scholar
Riley, L. W., Remis, R. S., Helgerson, S. D., McGee, H. B., Wells, J. G., Davis, B. R., Hebert, R. J., Olcott, E. S., Johnson, L. M., Hargrett, N. T., Blake, P. A. & Cohen, M. L. (1983). Hemorrhagic colitis associated with a rare Escherichia coli serotype. New England Journal of Medicine 308, 681–685.CrossRefGoogle ScholarPubMed
Schoonderwoerd, M., Clarke, R. C., Van Dreumel, T. A. & Rawluk, S. A. (1988). Colitis in calves: natural and experimental infection with a verotoxin-producing strain of Escherichia coli O 111. NM. Canadian Journal of Veterinary Research 54, 484–487.Google Scholar
Scotland, S. M., Rowe, B., Smith, H. R., Willshaw, G. A. & Gross, R. J. (1988). Verocytotoxin-producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. Journal of Medical Microbiology 25, 237–243.Google Scholar
Smith, H. W., Green, P. & Parsell, Z. (1983). Vero cell toxins in Escherichia coli and related bacteria: transfer by phage and conjugation and toxic action in laboratory animals, chickens and pigs. Journal of Medical Microbiology 129, 3121–3137.Google ScholarPubMed
Smith, H. W. & Linggood, M. A. (1971). The transmissible nature of enterotoxin production in a human enteropathogenic strain of Escherichia coli. Journal of Medical Microbiology 4, 301–305.CrossRefGoogle Scholar
Sherwood, D., Snodgrass, D. R. & O'Brien, A. D. (1985). Shiga-like toxin production from Escherichia coli associated with calf diarrhea. Veterinary Research 16, 217.Google Scholar
Spika, J. S., Parsons, J. E., Nordenbero, D. & Gunn, R. A. (1986). Hemolytic uremic syndrome and diarrhea associated with Escherichia coli O 157. H7 in a day-care center. Journal of Pediatrics 109, 287–291.CrossRefGoogle Scholar
Styliadis, S. & Barnum, D. (1984). Salmonella muenster infection in man and animals in the province of Ontario. Proceedings of the International Symposium on Salmonella. New Orleans. 200–208Google Scholar
Tzipori, S., Wachsmuth, I. K., Smithers, J. & Jackson, C. (1988). Studies in gnotobiotic piglets on non-O 157. H7. Escherichia coli serotypes isolated from patients with hemorrhagic colitis. Gastroenterology 94, 590–597.Google Scholar
Wells, J. G., Davis, B. K., Wachsmuth, I. K., Riley, L. W., Remis, R. S., Sokolow, A. & Morris, G. K. (1983). Laboratory investigation of hemmorhagic colitis outbreaks associated with a rare Escherichia coli serotype. Journal of Clinical Microbiology 18, 512–520.CrossRefGoogle Scholar
Wells, J. G., Shipman, L. D., Greene, K. D., Downess, F. P., Martin, M. L., Tauxe, R. V. & Wachsmuth, I. K. (1987). Isolation of Escherichia coli O 157. H7 and other Shiga-like/ verotoxin-producing E. coli from dairy cattle. Abstract LFE-4. International Symposium and Workshop on Verocytotoxin-producing Escherichia coli infections. Toronto, 12–1507.Google Scholar