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Molecular characterization of plasmids in Salmonella enteritidis phage types

Published online by Cambridge University Press:  19 October 2009

D. J. Brown ,
E. J. Threlfall ,
M. D. Hampton  and
B. Rowe
D. J. Brown
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61, Colindale Avenue, London NW9 5HT
E. J. Threlfall*
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61, Colindale Avenue, London NW9 5HT
M. D. Hampton
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61, Colindale Avenue, London NW9 5HT
B. Rowe
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61, Colindale Avenue, London NW9 5HT
*
†Author for correspondence.
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Summary

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Plasmids in selected type strains of 26 of the Salmonella enteritidis phage types have been characterized by restriction enzyme fingerprinting and by DNA–DNA hybridization with oligonucleotide probes for Salmonella plasmid virulence (Spv) genes. With one exception, the fingerprints of the 38 MDa plasmids studied were homogeneous but there was heterogeneity in the fingerprints of 59 MDa plasmids found in 4 of the type strains. However all 38 MDa and 59 MDa plasmids were related as was a 45 MDa plasmid identified in the type strain of phage type 19. A 3·5 kb fragment homologous to SpvC was conserved in Hind III digests of all 38 MDa and 59 MDa plasmids, and in the related 45 MDa plasmid. In contrast a 65 MDa plasmid found in the type strain of phage type 10 was not related to these three plasmid molecular weight groups and did not carry the SpvC gene.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 2 , April 1993 , pp. 209 - 216
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Anonymous. The microbiological safety of food, part 1. London: HMSO. 1990.Google Scholar
2.Ward, LR. de Sa, JDH. Rowe, B.A phage-typing scheme for Salmonella enteritidis. Epidemiol Infect 1987: 99: 291–4.Google ScholarPubMed
3.Threlfall, EJ, Howe, B, Ward, LR.Subdivision of Salmonella enteritidis phage types by plasmid profile typing. Epidemiol Infect 1989; 102: 459–65.Google ScholarPubMed
4.Helmuth, R, Stephan, R, Bunge, C, Hoog, B, Steinbeck, A, Bulling, E.Epidemiology of virulence-associated plasmids and outer membrane protein patterns with seven common Salmonella serotypes. Infect Immun 1985; 48: 175–82.Google ScholarPubMed
5.Popoff, MY, Minis, I, Coynault, CL, Lasselin, C, Pardon, P.Molecular relationships between virulence plasmids of Salmonella serotypes typhimurium and dublin and large plasmids of other Salmonella serotypes. Ann Microbiol Inst Pasteur 1984; 135A: 389–98.Google ScholarPubMed
6.Williamson, CM, Baird, GD, Manning, J.A common virulence region on plasmids from eleven serotypes of Salmonella. J Gen Microbiol 1988; 134: 975–82.Google ScholarPubMed
7.Poppe, C, Curtis, R III, Gulig, PA, Gyles, CL.Hybridization studies with a DNA probe derived from the virulence region of the GO MDAL plasmid of Salmonella typhimurium. Can J Vet Res 1989; 53: 378–84.Google Scholar
8.Norel, F, Pisano, M-R, Nicoli, J, Popoff, MY.. Nucleotide sequence of the plasmid-borne virulence gene mkfA encoding a 28 kDa polypeptide from Salmonella typhimurium. Res Microbiol Inst Pasteur 1989; 140: 263–5.Google ScholarPubMed
9.Pullinger, GD, Baird, GD, Williamson, CM, Lax, AJ.Nucleotide sequence of a plasmid gene involved in the virulence of salmonellas. Nuc Acid Res 1989; 17: 7983.Google ScholarPubMed
10.Matsui, H, Kawahara, K, Terakado, N, Danbara, H.Nucleotide sequence of a gene encoding a 29 kDa polypeptide in the mba region of the virulence plasmid, pKDSC50, of Salmonella choleraesuis. Nuc Acid Res 1990; 18: 2181–2.Google ScholarPubMed
11.Kado, CI, Liu, S-T.Rapid procedure for detection of large and small plasmids. J Bact 1981; 145: 1365–73.Google ScholarPubMed
12.Threlfall, EJ, Rowe, B, Ferguson, JL, Rowe, B.Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204c isolated in Britain. J Hyg 1986; 97: 419–26.Google ScholarPubMed
13.Macrina, FL, Kopecko, DJ, Jones, KR, Ayers, DJ, McCowan, SM.A multiple plasmidcontaining Eschcrichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1978; 1: 417–20.Google ScholarPubMed
14.Olsen, JE.An improved method for the rapid isolation of plasmid DNA from wild-type gram-negative bacteria for restriction profile analysis. Lett Appl Microbiol 1990; 10: 209–12.Google ScholarPubMed