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Development of a Laboratory Screening Technique, Based on Embryo Protein Synthesis, For the Assessment of High-Temperature Susceptibility During Germination of Sorghum bicolor

Published online by Cambridge University Press:  03 October 2008

H. J. Ougham  and
J. L. Stoddart
H. J. Ougham
Affiliation:
Department of Plant Biochemistry, Welsh Plant Breeding Station, Plus Gogerddan, Aberystwyth, Dyfed, SY23 3EB, Wales
J. L. Stoddart
Affiliation:
Department of Plant Biochemistry, Welsh Plant Breeding Station, Plus Gogerddan, Aberystwyth, Dyfed, SY23 3EB, Wales
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Summary

The capacity of sorghum lines to germinate at high soil temperatures (above 40°C) is clearly related to the temperature sensitivity of embryo protein synthesis. The development and optimization of a simple laboratory technique based on this correlation for the rapid large-scale screening of sorghum lines is described. Embryo-containing half seeds are incubated in the presence of 14C-labelled amino acids at different temperatures and the resulting radio-labelled proteins extracted and bound to nitrocellulose discs for counting. The extent to which incorporation of 14C-label into proteins is inhibited by elevated temperatures can be used to predict the likely temperature sensitivity of a given line during germination.

Type
Research Article
Information
Experimental Agriculture , Volume 21 , Issue 4 , October 1985 , pp. 343 - 355
Copyright
Copyright © Cambridge University Press 1985

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References

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