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The chromosomal integration of a β-lactamase gene derived from the P-type R-factor RP1 in Escherichia coli

Published online by Cambridge University Press:  14 April 2009

M. H. Richmond  and
R. B. Sykes
M. H. Richmond
Affiliation:
Department of Bacteriology, University of Bristol, University Walk, Bristol BS 8 1 TH
R. B. Sykes
Affiliation:
Department of Bacteriology, University of Bristol, University Walk, Bristol BS 8 1 TH

Extract

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The R-factor RP1, which carried the markers amp neo tet, confers resistance to penicillins by specifying the synthesis of Type IIIa β-lactamase. It was detected initially in a strain of Pseudomonas aeruginosa (Sykes & Richmond, 1970) and fragments spontaneously in Ps. aeruginosa strain Ps 18 to give clones which carry only the amp marker. Such clones are said to carry the element RP 1–1 since they are still capable of specifying the transfer of ampicillin and carbenicillin resistance to appropriate recipients in mating experiments. However, unlike lines carrying RP 1, they contain no detectable extrachromosomal DNA (Ingram et al. 1972), and the likely supposition is that the amp gene in these clones has become integrated into the bacterial chromosome together with the regions necessary to allow such strains to act as amp gene donors in mating experiments. It is probably cells of Ps. aeruginosa in which the amp gene has become integrated together with an RTF region that are responsible for the transfer of chromosomal markers reported by Holloway & Stanisich (1971).

Type
Research Article
Information
Genetics Research , Volume 20 , Issue 2 , October 1972 , pp. 231 - 237
Copyright
Copyright © Cambridge University Press 1972

References

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