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Recombination between the X and Y chromosomes and the Sxr region of the mouse

Published online by Cambridge University Press:  14 April 2009

Anne McLaren ,
Elizabeth Simpson ,
Colin E. Bishop ,
Michael J. Mitchell  and
Susan M. Darling
Anne McLaren*
Affiliation:
MRC Mammalian Development Unit, Wolfson House, 4 Stephenson Way, London NW1 2HE
Elizabeth Simpson
Affiliation:
Transplantation Biology Section, Clinical Research Centre, Watford Road, Harrow, Middlesex, HA1 3UJ
Colin E. Bishop
Affiliation:
Molecular Genetics Research Laboratory, Department of Obstetrics and Gynecology, Division of Reproductive Genetics, University of Tennessee, Memphis, TN 38105.
Michael J. Mitchell
Affiliation:
Molecular Genetics Research Laboratory, Department of Obstetrics and Gynecology, Division of Reproductive Genetics, University of Tennessee, Memphis, TN 38105.
Susan M. Darling
Affiliation:
MRC Mammalian Development Unit, Wolfson House, 4 Stephenson Way, London NW1 2HE
*
*Corresponding author.

Summary

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The Sxr (sex-reversed) region that carries a copy of the mouse Y chromosomal testis-determining gene can be attached to the distal end of either the Y or the X chromosome. During male meiosis, Sxr recombined freely between the X and Y chromosomes, with an estimated recombination frequency not significantly different from 50% in either direction. During female meiosis, Sxr recombined freely between the X chromosome to which it was attached and an X-autosome translocation. A male mouse carrying the original Sxra region on its Y chromosome, and the shorter Sxrb variant on the X, also showed 50% recombination between the sex chromosomes. Evidence of unequal crossing-over between the two Sxr regions was obtained: using five markers deleted from Sxrb, 3 variant Sxr regions were detected in 159 progeny (1·9%). Four other variants (one from the original cross and three from later generations) were presumed to have been derived from illegitimate pairing and crossing-over between Sxrb and the homologous region on the short arm of the Y chromosome. The generation of new variants throws light on the arrangement of gene loci and other markers within the short arm of the mouse Y chromosome.

Type
Research Article
Information
Genetics Research , Volume 60 , Issue 3 , December 1992 , pp. 175 - 184
Copyright
Copyright © Cambridge University Press 1992

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