Hostname: page-component-848d4c4894-ttngx Total loading time: 0 Render date: 2024-06-03T03:29:26.928Z Has data issue: false hasContentIssue false
  • English
  • Français

Concentrations of intermediate metabolites in the blood and hepatic tissues of pregnant and non-pregnant ewes

Published online by Cambridge University Press:  27 March 2009

I. D. Herriman ,
R. J. Heitzman ,
I. Priestley  and
G. S. Sandhu
I. D. Herriman
Affiliation:
Department of Functional Pathology, Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RG16 0NN
R. J. Heitzman
Affiliation:
Department of Functional Pathology, Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RG16 0NN
I. Priestley
Affiliation:
Department of Functional Pathology, Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RG16 0NN
G. S. Sandhu
Affiliation:
Department of Functional Pathology, Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RG16 0NN
Get access Rights & Permissions [Opens in a new window]

Summary

Six late pregnant and six non-pregnant Finnish Landrace × Dorset Horn ewes were fed a standard diet. Blood and liver samples were taken and the concentrations of the following metabolites measured: adenine monophosphate (AMP), adenine diphosphate (ADP), adenine triphosphate (ATP), citrate, 2-oxoglutarate, phosphoenol-pyruvate, pyruvate, lactate, 2·phosphoglycerate, 3·phosphoglycerate, malate, β·hydroxybutyrate, acetoacetate, glutamate, alanine, NAD+ and NADH. Comparing non-pregnant with pregnant ewes the liver of the pregnant animals had reduced concentrations of lactate, ADP, NAD+ and NADH, and in the blood there were reductions in lactate, pyruvate, lactate/pyruvate ratio and urea. The concentrations of the other metabolites of the pregnant ewes receiving adequate nutrition were little changed from those of the non-pregnant ewes. The significance of these findings is discussed.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 87 , Issue 2 , October 1976 , pp. 407 - 411
Copyright
Copyright © Cambridge University Press 1976

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adam, H. (1963). In Methods of Enzymatic Analysis (ed. Bergmeyer, H. V.), p. 539 and p. 573. London: Academic Press.Google Scholar
Atkinson, D. E. (1968). Citrate and the citrate cycle in the regulation of energy metabolism. In The Metabolic Role of Citrate (ed. Goodwin, T. W.), pp. 23–40. London: Academic Press.Google Scholar
Baird, G. D., Hibbitt, K. G., Hunter, G. D., Lund, P., Stubbs, M. & Krebs, H. A. (1968). Biochemical aspects of bovine ketosis. Biochemical Journal 107, 683–9.CrossRefGoogle ScholarPubMed
Baird, G. D. & Heitzman, R. J. (1970). Gluconeogenesis in the cow. The effects of a glucocorticoid on hepatic intermediary metabolism. Biochemical Journal 116, 865–74.CrossRefGoogle ScholarPubMed
Baird, G. D. & Heitzman, R. J. (1971). Modo of action of a glucocorticoid on bovine intermediary metabolism. Possible role in controlling hepatic ketogenesis. Biochimica et Biophysica Acta 252, 184–98.CrossRefGoogle Scholar
Baird, G. D., Heitzman, R. J. & Hibbitt, K. G. (1972). Effects of starvation on intermediary metabolism in the lactating cow, a comparison with metabolic changes occurring during bovine ketosis. Biochemical Journal 128, 1311–18.CrossRefGoogle ScholarPubMed
Baird, G. D., Symonds, H. W. & Ash, R. (1975). Some observations on metabolite production and utilization in vivo by the gut and liver of adult dairy cows. Journal of Agricultural Science, Cambridge 85, 281–96.CrossRefGoogle Scholar
Ballard, F. J., Hanson, R. W., Kronfeld, D. S. & Raggi, F. (1968). Metabolic changes in liver associated with spontaneous ketosis and starvation in cows. Journal of Nutrition 95, 160–72.CrossRefGoogle Scholar
Garber, A. J., Ballard, F. J. & Hanson, R. W. (1972). The significance of mitochondrial phosphoenolpyruvate formation in the regulation of gluconeogenesis in guinea pig liver. In Energy Metabolism and Regulation of Metabolic Processes in Mitochondria (ed. Mehlman, M. A. and Hanson, R. W.), pp. 109–35. New York and London: Academic Press.CrossRefGoogle Scholar
Greenbaum, A. L., Gumaa, K. A. & McLean, P. (1971). The distribution of hepatic metabolites and the control of the pathways of carbohydrate metabolism in animals of different dietary and hormonal status. Archives of Biochemistry and Biophysics 143, 617–63.CrossRefGoogle ScholarPubMed
Heitzman, R. J. & Baird, G. D. (1970). Metabolic control in the cow by glucocorticosteroids. Journal of Endocrinology 48, lxxvi–lxxvii.Google ScholarPubMed
Heitzman, R. J., Hibbitt, K. G. & Mather, J. (1971). The effects of thyroxine on hepatic gluconeogenesis and ketogenesis in dairy cows. European Journal of Biochemistry 21, 411–15.CrossRefGoogle ScholarPubMed
Katz, M. L. & Bergman, E. N. (1969). Hepatic and portal metabolism of glucose, free fatt yacids and ketone bodies in sheep. American Journal of Physiology 216, 953–60.CrossRefGoogle Scholar
Klingenberg, M. (1963). In Methods of Enzymatic Analysis (ed. Bergmeyer, H. V.), pp. 528–31. London: Academic Press.Google Scholar
Lindsay, D. B. (1970). Carbohydrate metabolism in ruminants. In Physiology of Digestion and Metabolism in the Ruminant (ed. Phillipson, A. T.), pp. 438–51. Oriel Press: Newcastle-upon-Tyne.Google Scholar
National Research Council (1972). Nutrient Requirements of Sheep. National Academy of Science, Washington D.C.Google Scholar
Patterson, D. S. P., Burns, K. N., Cunningham, N. F., Herbert, C. N. & Saba, N. (1964). Plasma concentrations of glucose and non-esterified fatty acids (NEFA) in the pregnant and lactating ewe and the effect of dietary restriction. Journal of Agricultural Science, Cambridge 62, 253–62.CrossRefGoogle Scholar
Reid, R. L. (1968). The physiopathology of under-nourishment in pregnant sheep, with particular reference to pregnancy toxaemia. Advances in Veterinary Science 12, 167–238.Google Scholar
Reilly, P. E. B. & Ford, E. J. H. (1974). The effect of betamethasone on glucose production and on gluconeogenesis from amino acids in sheep. Journal of Endocrinology 60, 455–61.CrossRefGoogle ScholarPubMed
Start, C. & Newsholme, E. A. (1968). The effects of starvation and alloxan-diabetes on the contents of citrate and other metabolic intermediates in rat liver. Biochemical Journal 107, 411–15.CrossRefGoogle ScholarPubMed
Taylor, P. H., Wallace, J. C. & Keech, D. B. (1971). Gluconeogenic enzymes in sheep liver. Intracellular localisation of pyruvate carboxylase and phosphoenolpyruvate carboxykinase in normal, fasted and diabetic sheep. Biochimica et Biophysica Acta 237, 179–91.CrossRefGoogle ScholarPubMed
Wieland, O. (1968). Ketogenesis and its regulation. In Advances in Metabolic Disorders (ed. Levine, R. and Luft, R.), pp. 1–47. New York: Academic Press.Google Scholar
Williamson, D. H., Lund, P. & Krebs, H. A. (1967). The redox state of free nicotinamide adenine dinucleotide in the cytoplasm and mitochondria of rat liver. Biochemical Journal 103, 514–27.CrossRefGoogle ScholarPubMed
Williamson, D. H., Veloso, D., Ellington, E. V. & Krebs, H. A. (1969). Changes in the concentrations of hepatic metabolites on administration of dihydroxyacetone or glycerol to starved rats and the relationship to the control of ketogenesis. Biochemical Journal 114, 575–84.CrossRefGoogle ScholarPubMed