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Detection of lytic phage infecting flavour-producing strain of Lacticaseibacillus paracasei in the dairy effluents of Kerala
Published online by Cambridge University Press: 21 June 2023
Abstract
The performance of the starter culture is a critical factor that decides the quality of fermented milk. Dahi is a fermented milk product popular in India made using a mixed starter culture of lactic acid bacteria comprising acid and flavour producers. The prevalence of bacteriophages in the dairy environment can critically affect the activity of these starter cultures resulting in starter failure. As there is little information available on the occurrence of bacteriophages in the dairy environment of Kerala, this research communication examines the presence of lytic bacteriophages acting against three potential flavour-producing strains of Lacticaseibacillus paracasei (Lc. paracasei). Dairy effluent samples were screened for the presence of phages against the strains of Lc. paracasei by the multiple host enrichment method. Plates showing clearance zone in spot assay were confirmed for the presence of phages by double-layer agar assay. The plaques obtained in the double-layer agar assay were purified for further identification by next-generation sequencing. A bacteriophage infecting one of the three strains of Lc. paracasei was detected by the plaque assay and the blast annotation of the bacteriophage sequence found 86.05% similarity of the phage to Siphoviridae family. The study endorses the need for monitoring phages in the dairy environment to control phage-related starter failure in the state of Kerala.
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- Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation
References
Bengoa, AA, Dardis, C, Garrote, GL and Abraham, AG (2021) Health-promoting properties of Lacticaseibacillus paracasei: a focus on Kefir isolates and exopolysaccharide-producing strains. Foods 10, 2239.Google Scholar
Feyereisen, M, Mahony, J, Lugli, GA, Ventura, M, Neve, H, Franz, CM, Noben, JP, O'Sullivan, T and Van Sinderen, D (2019) Isolation and characterization of Lactobacillus brevis phages. Viruses 11, 393.Google Scholar
Kot, W, Vogensen, FK, Sørensen, SJ and Hansen, LH (2014) DPS – a rapid method for genome sequencing of DNA-containing bacteriophages directly from a single plaque. Journal of Virology Methods 196, 152–156.Google Scholar
Mahony, J, Murphy, J and van Sinderen, D (2012) Lactococcal 936-type phages and dairy fermentation problems: from detection to evolution and prevention. Frontiers in Microbiology 3, 335.CrossRefGoogle ScholarPubMed
Marcó, MB, Moineau, S and Quiberoni, A (2012) Bacteriophages and dairy fermentations. Bacteriophage 2, 149–158.Google Scholar
Mercanti, DJ, Rousseau, GM, Capra, ML, Quiberoni, A, Tremblay, DM, Labrie, SJ and Moineau, S (2016) Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei. Applied and Environmental Microbiology 82, 95–105.CrossRefGoogle ScholarPubMed
Moineau, S, Pandian, S and Klaenhammer, TR (1994). Evolution of a lytic bacteriophage via DNA acquisition from the Lactococcus lactis chromosome. Applied and Environmental Microbiology 60, 1832–1841.CrossRefGoogle ScholarPubMed
Sunthornthummas, S, Doi, K, Rangsiruji, A, Sarawaneeyaruk, S and Pringsulaka, O (2017) Isolation and characterization of Lactobacillus paracasei LPC and phage ΦT25 from fermented milk. Food Control 73, 1353–1361.CrossRefGoogle Scholar
Vaiyapuri, M, Raveendran, K, George, I, Gundubilli, D, Sivam, V, Krishnan, SG, George, JC, Mothadaka, MP, Nagarajarao, RC and Badireddy, MR (2021). Comparison of single and multi-host enrichment approach for harnessing lytic phages against antimicrobial-resistant E. coli: repurposing the enrichment step. Biologia 76, 1041–1052.CrossRefGoogle Scholar
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