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Probing of alpha, beta and kappa-caseins polymorphic variants in Gangatiri cow milk with the use of polyacrylamide gel electrophoresis and HRAMS

Published online by Cambridge University Press:  17 February 2023

Manish Kumar Singh ,
Arvind Kumar ,
Ramadevi Nimmanapalli  and
Ankita Hooda
Manish Kumar Singh
Affiliation:
Department of Dairy Science and Food Technology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
Arvind Kumar*
Affiliation:
Department of Dairy Science and Food Technology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
Ramadevi Nimmanapalli
Affiliation:
Faculty of Veterinary and Animal Sciences, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
Ankita Hooda
Affiliation:
Department of Dairy Science and Food Technology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
*
Author for correspondence: Arvind Kumar, Email: arvind1@bhu.ac.in
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Abstract

This research communication aimed to probe the genetic polymorphisms of alpha, beta, and kappa caseins in Gangatiri cows (an indigenous Indian cattle). Detection of variants has received considerable research interest in the dairy industry and animal breeding in recent years as a source of good quality protein, but also of bioactive peptides that may be linked to health implications. The polymorphic nature of casein fractions and their association with milk production traits, composition, and quality also attracted several efforts in evaluating the allelic distribution of different casein locus as a potential dairy trait marker. Molecular techniques of polyacrylamide gel electrophoresis and high-resolution accurate mass-spectrometry have been applied to this probe. Sequence analysis of different casein types in the cows showed the presence of four specific variants.

Keywords

Alpha-caseinbeta-caseinGangatiri cowkappa-caseinmass spectrometrypolyacrylamide gel electrophoresis
Type
Research Article
Information
Journal of Dairy Research , Volume 90 , Issue 1 , February 2023 , pp. 54 - 57
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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