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Selective enumeration of propionibacteria in Emmental-type cheese using Petrifilm™ Aerobic Count plates added to Lithium Glycerol broth

Published online by Cambridge University Press:  23 April 2013

Rosângela de Freitas ,
Lívia M. Pinheiro Luiz ,
Maura Pinheiro Alves ,
Florence Valence-Bertel ,
Luís Augusto Nero  and
Antônio Fernandes de Carvalho
Rosângela de Freitas
Affiliation:
Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, MG, Brasil
Lívia M. Pinheiro Luiz
Affiliation:
Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, MG, Brasil
Maura Pinheiro Alves
Affiliation:
Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, MG, Brasil
Florence Valence-Bertel
Affiliation:
Science et Technologie du Lait e de l'Œuf, CIRM-BIA, Institut National de la Recherche Agronomique, Rennes, France
Luís Augusto Nero*
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, MG, Brasil
Antônio Fernandes de Carvalho*
Affiliation:
Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, MG, Brasil
*
*For correspondence; e-mail: antoniofernandes@ufv.br, nero@ufv.br
*For correspondence; e-mail: antoniofernandes@ufv.br, nero@ufv.br
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Abstract

Propionibacteria derived from dairy products are relevant starter cultures for the production of Swiss and Emmental-type cheeses, and the monitoring of which is mandatory for proper quality control. This study aimed to evaluate an alternative procedure to enumerate propionibacteria, in order to develop a reliable and practical methodology to be employed by dairy industries. 2,3,5-triphenyltetrazolium chloride (TTC) inhibitory activity was tested against five reference strains (CIRM 09, 38, 39, 40 and 116); TTC at 0·0025% (w/v) was not inhibitory, with the exception of one strain (CIRM 116). Subsequently, the four TTC-resistant strains, three commercial starter cultures (PS-1, PB-I, and CHOO) and twelve Emmental-type cheese samples were subjected to propionibacteria enumeration using Lithium Glycerol (LG) agar, and Petrifilm™ Aerobic Count (AC) plates added to LG broth (anaerobic incubation at 30 °C for 7 d). Petrifilm™ AC added to LG broth presented high counts than LG agar (P<0·05) for only two reference strains (CIRM 39, and 40) and for all commercial starter cultures. Cheese sample counts obtained by both procedures did not show significant differences (P<0·05). Significant correlation indexes were observed between the counts recorded by both methods (P<0·05). These results demonstrate the reliability of Petrifilm™ AC plates added to LG broth in enumerating select Propionibacterium spp., despite some limitations observed for specific commercial starter cultures.

Keywords

Propionic bacteriaPetrifilm™ ACcheeseEmmental
Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 3 , August 2013 , pp. 270 - 275
Copyright
Copyright © Proprietors of Journal of Dairy Research 2013 

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