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Effect of maternal and postnatal cocoa supplementation on testicles of adult Wistar rats

Published online by Cambridge University Press:  20 July 2020

Juliana Lima ,
Letícia Cardoso ,
Gabrielle Rocha ,
Caroline Fernandes-Santos ,
Diogo de Souza ,
Francisco Jose Sampaio  and
Bianca Gregorio Open the ORCID record for Bianca Gregorio [Opens in a new window]
Juliana Lima
Affiliation:
Urogenital Research Unit, Department of Anatomy, UERJ, Rio de Janeiro, Brazil
Letícia Cardoso
Affiliation:
Faculty of Nutrition Emília de Jesus Ferreiro, Department of Nutrition and Dietetics, UFF, Niterói, Brazil
Gabrielle Rocha
Affiliation:
Faculty of Nutrition Emília de Jesus Ferreiro, Department of Nutrition and Dietetics, UFF, Niterói, Brazil
Caroline Fernandes-Santos
Affiliation:
Laboratory Multiuser of Biomedical Research, Department of Basic Sciences, UFF, Nova Friburgo, Brazil
Diogo de Souza
Affiliation:
Urogenital Research Unit, Department of Anatomy, UERJ, Rio de Janeiro, Brazil
Francisco Jose Sampaio
Affiliation:
Urogenital Research Unit, Department of Anatomy, UERJ, Rio de Janeiro, Brazil
Bianca Gregorio*
Affiliation:
Urogenital Research Unit, Department of Anatomy, UERJ, Rio de Janeiro, Brazil
*
Address for correspondence: Bianca Gregorio, Urogenital Research Unit, Department of Anatomy, Biomedical Center, State University of Rio de Janeiro, Av. 28 de setembro 87 (fds), 20551-030Rio de Janeiro, RJ, Brazil. Email: biancamgregorio.uerj@gmail.com
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Abstract

Early weaning can lead to changes in the morphology of organs in adulthood, and the consumption of functional foods during lactation and postnatal life is believed to prevent these changes. However, it is not known if early weaning affects testicular morphology and if the use of cocoa can prevent that. We studied the effects of maternal and postnatal supplementation of cocoa powder on the testicular morphology of early weaned adult rats. The animals were divided into four groups (n = 6 each), control group, cocoa control group, early weaning (EW) group, and cocoa early weaning (EWCa) group, and were analyzed for 90 d, after which they were euthanized. The animals from the EW group showed a reduction in the tubular diameter and height of the seminiferous epithelium, a decrease in epithelial surface density (Sv), and an increase in the lumen and proper tunic. However, the animals from the EWCa group showed an increase in the diameter and height of the epithelium, an increase in the epithelium Sv, and a decrease in the lumen and the proper tunic. The early weaning promotes morphological changes in the testicles; however, supplementation with cocoa powder can preserve the testicular histoarchitecture.

Keywords

early weaningtesticlefetal programmingcocoa
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 12 , Issue 3 , June 2021 , pp. 436 - 442
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Copyright
© The Author(s), 2020. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease

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