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An integrative taxonomy study reveals a rare new species of the genus Creptotrema (Trematoda: Allocreadiidae) in an endangered frog in South America

Published online by Cambridge University Press:  11 March 2024

E.P. Alcantara Open the ORCID record for E.P. Alcantara [Opens in a new window] ,
M.B. Ebert Open the ORCID record for M.B. Ebert [Opens in a new window] ,
C. Ferreira-Silva Open the ORCID record for C. Ferreira-Silva [Opens in a new window] ,
L.R. Forti Open the ORCID record for L.R. Forti [Opens in a new window] ,
D.H. Morais Open the ORCID record for D.H. Morais [Opens in a new window] ,
G. Pérez-Ponce de León Open the ORCID record for G. Pérez-Ponce de León [Opens in a new window]  and
R. J. Silva Open the ORCID record for R. J. Silva [Opens in a new window]
E.P. Alcantara
Affiliation:
Universidade Estadual Paulista (UNESP), Instituto de Biosciências, Setor de Parasitologia, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo 18618-689, Brazil
M.B. Ebert
Affiliation:
Universidade Estadual Paulista (UNESP), Instituto de Biosciências, Setor de Parasitologia, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo 18618-689, Brazil
C. Ferreira-Silva*
Affiliation:
Universidade Federal do Ceará (UFC), Departamento de Biologia, Centro de Ciências, Av. Mister Hull, s/n, CEP 60455-760, Fortaleza, Ceará, Brazil
L.R. Forti
Affiliation:
Departamento de Biociências, Universidade Federal Rural do Semi-Árido (UFERSA), Av. Francisco Mota, 572 - Bairro Costa e Silva, 59625-900, Mossoró – Rio Grande do Norte, Brazil
D.H. Morais
Affiliation:
Universidade Federal de Uberlândia (UFU), Instituto de Ciências Agrárias, LMG-746, Km 1, Monte Carmelo, 38500-000, MG, Brazil
G. Pérez-Ponce de León
Affiliation:
Escuela Nacional de Estudios Superiores Unidad Mérida (ENES)-UNAM, Km 4.5 Carretera Mérida-Tetiz, Ucú, Yucatán, Mexico
R. J. Silva
Affiliation:
Universidade Estadual Paulista (UNESP), Instituto de Biosciências, Setor de Parasitologia, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo 18618-689, Brazil
*
Corresponding author: C. Ferreira-Silva; Email: cristiannafsilva@gmail.com
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Abstract

During an ecological study with a near-endangered anuran in Brazil, the Schmidt’s Spinythumb frog, Crossodactylus schmidti Gallardo, 1961, we were given a chance to analyze the gastrointestinal tract of a few individuals for parasites. In this paper, we describe a new species of an allocreadiid trematode of the genus Creptotrema Travassos, Artigas & Pereira, 1928, which possesses a unique trait among allocreadiids (i.e., a bivalve shell-like muscular structure at the opening of the ventral sucker); the new species represents the fourth species of allocreadiid trematode parasitizing amphibians. Besides, the new species is distinguished from other congeners by the combination of characters such as the body size, ventral sucker size, cirrus-sac size, and by having small eggs. DNA sequences through the 28S rDNA and COI mtDNA further corroborated the distinction of the new species. Phylogenetic analyses placed the newly generated sequences in a monophyletic clade together with all other sequenced species of Creptotrema. Genetic divergences between the new species and other Creptotrema spp. varied from 2.0 to 4.2% for 28S rDNA, and 15.1 to 16.8% for COI mtDNA, providing robust validation for the recognition of the new species. Even though allocreadiids are mainly parasites of freshwater fishes, our results confirm anurans as hosts of trematodes of this family. Additionally, we propose the reallocation of Auriculostoma ocloya Liquin, Gilardoni, Cremonte, Saravia, Cristóbal & Davies, 2022 to the genus Creptotrema. This study increases the known diversity of allocreadiids and contributes to our understanding of their evolutionary relationships, host–parasite relationships, and biogeographic history.

Keywords

AnuraDigeneaNeotropical region28S rDNACOI mtDNA
Type
Research Paper
Information
Journal of Helminthology , Volume 98 , 2024 , e23
Copyright
© The Author(s), 2024. Published by Cambridge University Press

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