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Characterization of adult Dicrocoelium dendriticum by isoelectric focusing

Published online by Cambridge University Press:  05 June 2009

R. Campo ,
M.Y. Manga-González ,
C. González-Lanza ,
D. Rollinson  and
H. Sandoval
R. Campo
Affiliation:
Departamento de Sistemas de Producción y Sanidad Animal, Estación Agrícola Experimental, Consejo Superior de Investigaciones Científicas (CSIC), Apartado 788, 24080 León, Spain
M.Y. Manga-González*
Affiliation:
Departamento de Sistemas de Producción y Sanidad Animal, Estación Agrícola Experimental, Consejo Superior de Investigaciones Científicas (CSIC), Apartado 788, 24080 León, Spain
C. González-Lanza
Affiliation:
Departamento de Sistemas de Producción y Sanidad Animal, Estación Agrícola Experimental, Consejo Superior de Investigaciones Científicas (CSIC), Apartado 788, 24080 León, Spain
D. Rollinson
Affiliation:
The Natural History Museum, Cromwell Road, London, SW7 5BD, UK
H. Sandoval
Affiliation:
Departamento de Sistemas de Producción y Sanidad Animal, Estación Agrícola Experimental, Consejo Superior de Investigaciones Científicas (CSIC), Apartado 788, 24080 León, Spain
*
*Author for correspondence. Fax: 34 87 317161
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Abstract

Water soluble extracts of 3131 adult specimens of Dicrocoelium dendriticum from cattle, sheep and goats, mainly from León province, were analysed by isoelectric focusing in thin-layer polyacrylamide gels. Activity of the following enzymes was studied: lactate dehydrogenase (LDH, EC 1.1.1.27), glucose phosphate isomerase (GPI, EC 5.3.1.9), phosphoglucomutase (PGM, EC 2.7.5.1), acid phosphatase (AcP, EC 3.1.3.2), α-glycerophosphate dehydrogenase (α-GPDH, EC 1.1.1.8), hydroxybutyrate dehydrogenase (HBDH, EC 1.1.1.30) and malate dehydrogenase (MDH, 1.1.1.37). Five distinct enzyme types were recognized for LDH (pH range 6.30–7.13), GPI (pH 6.13–6.80) and PGM (pH 6.20–6.60) whereas AcP showed three different patterns (pH 5.70–5.92). Weak and diffuse activity was detected for MDH (pH 4.8–6.2) and no activity was observed for α-GPDH and HBDH. In general, little phenotypic variation was observed between worms recovered from a single host, between those from hosts of the same species and between those from hosts of different species, although some enzyme types were found in some animals but not others. Nevertheless, it must be taken into account that most parasites came from sheep and also from a relatively small area in north-west Spain.

Type
Research Papers
Information
Journal of Helminthology , Volume 72 , Issue 2 , June 1998 , pp. 109 - 116
Copyright
Copyright © Cambridge University Press 1998

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