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Diagnosis of the strongyloid nematode Strongyloides venezuelensis in experimentally infected rats

Published online by Cambridge University Press:  14 July 2015

P.D. Marques ,
F.M. Malta ,
D.M.C.L. Meisel ,
M.A. Corral ,
J.R. Pinho ,
J.M. Costa-Cruz ,
P.P. Chieffi ,
R.C.B. Gryschek  and
F.M. Paula
P.D. Marques
Affiliation:
Instituto de Medicina Tropical de São Paulo
F.M. Malta
Affiliation:
Laboratório de Gastroenterologia e Hepatologia Tropical, Hospital das Clínicas da Faculdade de Medicina
D.M.C.L. Meisel
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil
M.A. Corral
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil
J.R. Pinho
Affiliation:
Laboratório de Gastroenterologia e Hepatologia Tropical, Hospital das Clínicas da Faculdade de Medicina
J.M. Costa-Cruz
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
P.P. Chieffi
Affiliation:
Faculdade de Ciências Médicas, Santa Casa, São Paulo, Brazil
R.C.B. Gryschek
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil
F.M. Paula*
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil
*
* E-mail: fabiana.paula@hc.fm.usp.br
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Abstract

Strongyloides venezuelensis is an intestinal nematode of rats, frequently used as a model for studying human and animal strongyloidiasis. In the present study, we evaluated parasitological, serological and molecular methods for the diagnosis of experimental S. venezuelensis in rats, Rattus norvegicus. Blood and faecal samples were collected and analysed up to 60 days post infection (pi) with adult worm recovery occurring from 5 to 45 days pi. Using an enzyme-linked immunosorbent assay (ELISA), serum levels of IgG antibodies increased up to 28 days pi, thereafter decreasing by day 60 pi. Polymerase chain reaction (PCR) assays detected S. venezuelensis DNA in faecal samples of rats from 5 to 21 days pi. The present study therefore represents the first step towards improving the diagnosis of experimental strongyloidiasis.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 4 , July 2016 , pp. 422 - 427
Copyright
Copyright © Cambridge University Press 2015 

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References

Baek, B.K., Islamb, M.K., Kimb, B.S., Limb, C.W., Hur, J., Oluoch, A.O., Kima, C.H. & Kakoma, I. (2003) Characterization of the protective response against a homologous challenge infection with Strongyloides venezuelensis in rats. Veterinary Parasitology 113, 217–227.Google Scholar
Chiuso-Minicucci, F., Marra, N.M., Zornella-Pezavento, S.F., França, T.G., Ishikawa, L.L., Amarante, M.R.V., Amarante, A.F.T. & Sartori, A. (2010) Recovery from Strongyloides venezuelensisinfection in Lewis rats is associated with a strong Th2 response. Parasite Immunology 32, 74–78.Google Scholar
Costa-Cruz, J.M., Bullamah, C.B., Gonçalves-Pires, M.R., Campos, D.M. & Vieira, M.A. (1997) Cryo-microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis. Revista do Instituto de Medicina Tropical de São Paulo 39, 313–317.Google Scholar
Dorris, M., Viney, M.E. & Blaxter, M.L. (2002) Molecular phylogenetic analysis of the genus Strongyloides and related nematodes. International Journal of Parasitology 32, 1507–1517.CrossRefGoogle ScholarPubMed
Feliciano, N.D., Gonzaga, H.T., Gonçalves-Pires, M.R.F., Gonçalves, A.L.R., Rodrigues, R.M., Ueta, M.T. & Costa-Cruz, J.M. (2010) Hydrophobic fractions from Strongyloides venezuelensis for use in the human immunodiagnosis of strongyloidiasis. Diagnostic Microbiology and Infectious Disease 67, 153–161.CrossRefGoogle ScholarPubMed
Gonçalves, A.L., Silva, C.V., Ueta, M.T. & Costa-Cruz, J.M. (2012) Antigen, antibody and immune complex detection in serum samples from rats experimentally infected with Strongyloides venezuelensis . Experimental Parasitology 130, 205–208.Google Scholar
Gonzaga, H.T., Ribeiro, V.S., Feliciano, N.D., Manhani, M.N., Silva, D.A., Ueta, M.T. & Costa-Cruz, J.M. (2011) IgG avidity in differential serodiagnosis of human strongyloidiasis active infection. Immunology Letters 139, 87–92.Google Scholar
Gordon, H.M. & Whitlock, H.V.A. (1939) New technique for counting nematode eggs in sheep faeces. Journal of Scientific and Industrial Research 12, 50–52.Google Scholar
Grove, D.I. (1996) Human strongyloidiasis. Advances in Parasitology 38, 251–309.Google Scholar
Kramme, S., Nicole, N., Soblik, H., Erttmann, K., Tannich, E., Fleischer, B., Pannig, M. & Brattig, N. (2011) Novel real-time PCR for the universal detection of Strongyloides species. Journal of Medical Microbiology 60, 454–458.Google Scholar
Looss, A. (1911) The anatomy and life history of Ancylostoma duodenale Dub. A monography. Part II. The development in the free state (translated from the German by M. Bemhard). Records of the School of Medicine, Egyptian Ministry of Education 4, 163–613.Google Scholar
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.Google Scholar
Machado, E.R., Ueta, M.T., Gonçalves-Pires, M.R.F., Oliveira, J.B., Faccioli, L.H. & Costa-Cruz, J.M. (2003) Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay. Memórias do Instituto Oswaldo Cruz 98, 849–851.Google Scholar
Marra, N.M., Chiuso-Minicucci, F., Machado, G.C., Zorzella-Pezavento, S.F., França, T.G., Ishikawa, L.L., Amarante, A.F.T., Sartori, A. & Amarante, M.R.V. (2010) Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats. Memórias do Instituto Oswaldo Cruz 105, 57–61.CrossRefGoogle ScholarPubMed
Marra, N.M., Chiuso-Minicucci, F., Machado, G.C., Zorzella-Pezavento, S.F., França, T.G., Ishikawa, L.L., Amarante, A.F.T., Sartori, A. & Amarante, M.R.V. (2011) Migratory route of Strongyloides venezuelensis in Lewis rats: comparison of histological analyses and PCR. Experimental Parasitology 127, 334–339.Google Scholar
Mejia, R. & Nutman, T.B. (2012) Screening, prevention, and treatment for hyperinfection syndrome and disseminated infections caused by Strongyloides stercoralis . Current Opinion in Infectious Diseases 25, 458–463.Google Scholar
Nakai, E.S. & Amarante, A.F.T. (2001) Infecção experimental de camundongos (Mus musculus) e ratos (Rattus norvegicus) com Strongyloides venezuelensis . Revista Brasileira de Parasitologia Veterinária 10, 1–6.Google Scholar
Olsen, A., van Lieshout, L., Marti, H., Polderman, T., Polman, K., Steinmann, P., Stothard, R., Thybo, S., Verweij, J.J. & Magnussen, P. (2009) Strongyloidiasis – the most neglected of the neglected tropical diseases? Transactions of the Royal Society of Tropical Medicine and Hygiene 103, 967–972.Google Scholar
Paula, F.M. & Costa-Cruz, J.M. (2011) Epidemiological aspects of strongyloidiasis in Brazil. Parasitology 138, 1331–1340.Google Scholar
Paula, F.M., Sitta, R.B., Malta, F.M., Gottardi, M., Corral, M.A., Gryschek, R.C.B. & Chieffi, P.P. (2013) Parasitological and molecular diagnosis in experimental Strongyloides venezuelensis infection. Revista do Instituto de Medicina Tropical de São Paulo 55, 141–143.Google Scholar
Rugai, E., Mattos, T. & Brisola, A.P. (1954) A new technique for the isolation of nematode larvae from faeces; modification of Baermann's method. Revista do Instituto Adolfo Lutz 14, 5–8.Google Scholar
Sitta, R.B., Malta, F.M., Pinho, J.R., Chieffi, P.P., Gryschek, R.C. & Paula, F.M. (2014) Conventional PCR for molecular diagnosis of human strongyloidiasis. Parasitology 141, 716–721.Google Scholar
Sopelete, M.C. (2005) Métodos de análises em estudos sobre diagnóstico. pp. 203–223 in Mineo, J.R., Silva, D.A.O., Sopelete, M.C., Leal, G.S., Vidigal, L.H.G., Tápia, L.E.R. & Bacchin, M.I. (Eds) Pesquisa na área biomédica: do planejamento à publicação. Uberlândia, EDUFU.Google Scholar
ten Hove, R.J., van Esbroeck, M., Vervoort, T., van den Ende, T., van Lieshout, L. & Verweij, J.J. (2009) Molecular diagnostics of intestinal parasites in returning travelers. European Journal of Clinical Microbiology and Infectious Diseases 28, 1045–1053.Google Scholar
Verweij, J.J., Canales, M., Polman, K., Ziem, J., Brienen, E.A.T., Polderman, A.M. & van Lieshout, L. (2009) Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR. Transactions of the Royal Society of Tropical Medicine and Hygiene 103, 342–346.Google Scholar