Hostname: page-component-848d4c4894-wzw2p Total loading time: 0 Render date: 2024-06-02T09:19:40.001Z Has data issue: false hasContentIssue false
  • English
  • Français

The in vitro effect of Khawia sinensis on leucocyte activity in carp (Cyprinus carpio)

Published online by Cambridge University Press:  05 June 2009

N.J. Morley  and
D. Hoole
N.J. Morley
Affiliation:
Parasitology Research Group, Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK
D. Hoole*
Affiliation:
Parasitology Research Group, Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK
*
*Author for correspondence.
Get access Rights & Permissions [Opens in a new window]

Abstract

The interactions between Khawia sinensis (Cestoda: Caryophyllidea), a pathogenic tapeworm of carp, and host leucocytes has been investigated in vitro by monitoring blastogenesis in pronephric, splenic and thymic lymphocytes and apoptosis of pronephric leucocytes. Both parasite homogenate and excretory/secretory (E/S) products were found to suppress lymphocyte blastogenesis at high concentrations (346.6–98.8 μg ml−1) whilstcell stimulation occurred at low concentrations (49.4–19.8 μg ml−1). This differential affect on leucocyte activity may not be associated with apoptosis in vitro as the parasite E/S products did not significantly affect programmed cell death as monitored by acridine orange and DNA analysis. The parasite effect on leucocyte activity is discussed and its relationship to host susceptibility is evaluated.

Type
Research Papers
Information
Journal of Helminthology , Volume 71 , Issue 1 , March 1997 , pp. 47 - 52
Copyright
Copyright © Cambridge University Press 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alford, P.B., Tomasso, J.R., Bodine, A.B. & Kendall, C. (1994) Apoptotic death of peripheral leucocytes in channel catfish: effects of confinement-induced stress. Journal of Aquatic Animal Health 6, 64–69.2.3.CO;2>CrossRefGoogle Scholar
Burger, C.J., Rikihisa, Y. & Young, C.L. (1986) Taenia taeniaformis: Inhibition of mitogen induced proliferation and interlukin-2 production in rat splenocytes by larval in vitro product. Experimental Parasitology 62, 216–222.CrossRefGoogle Scholar
Chubb, J.C. & Yeomans, W.E. (1995) Khawia sinensis Hsu, 1935 (Cestoda: Caryophyllidea), a tapeworm new to the British Isles: a threat to carp fisheries? Fisheries Management and Ecology 2, 263–277.CrossRefGoogle Scholar
Davydov, V.G. & Poddubnaya, L.G. (1988) [Functional morphology of the frontal and uterine glands of some Caryophyllidea.] Parazitologiya 22, 449–457 [in Russian].Google ScholarPubMed
Goldstein, P., Ojcius, D.M. & Young, J.D.E. (1991) Cell death mechanisms and the immune system. Immunological Reviews 121, 29–65.CrossRefGoogle Scholar
Greenlee, A.R., Brown, R.A. & Ristow, S.S. (1991) Nonspecific cytotoxic cells of rainbow trout (Oncorhynchus mykiss) kill Yac-1 targets by both necrotic and apoptic mechanisms. Developmental and Comparative Immunology 15, 153–164.CrossRefGoogle ScholarPubMed
Hammerberg, B. & Williams, J.F. (1978) Physicochemical characterization of complement interacting factors from Taenia taeniaeformis. Journal of Immunology 120, 1039–1045.CrossRefGoogle ScholarPubMed
Judson, D.T., Dixon, J.B. & Skerritt, G.C. (1987) Occurrence and biochemical characteristics of cestode mitogens. Parasitology 94, 151–160.CrossRefGoogle ScholarPubMed
Kapustina, N.I. (1978) [Host-parasite relationships in the system Khawia sinensis–carp in low intensity infection.] Trudy Vsesoyuznogo Nauchno-Issledovatel'skogo Instituta Prudovogo Rybnogo Khozyaistva (Parazity, bolezni ryb i ikh parazity) 27, 75–87 [in Russian].Google Scholar
Lopes, M.F., Daveiga, V.F., Santos, A.R., Fonseca, M.E.F. & Dosreis, G.A. (1995) Activation-induced CD4(+) T-cell death by apoptosis in experimental Chagas disease. Journal of Immunology 154, 744–752.CrossRefGoogle ScholarPubMed
Meseguer, J.M., Esteban, M.A. & Mulero, V. (1996) Nonspecific cell-mediated cytotoxicity in the seawater teleosts (Sparus aurata and Dicentrarchus labrax): ultrastructural study of target cell death mechanisms. The Anatomical Record 244, 499–505.3.0.CO;2-Q>CrossRefGoogle ScholarPubMed
Moore, K.J., Turco, S.J. & Matlashewski, G. (1994) Leishmania donovani infection enhances macrophage viability in the absence of exogenous growth-factor. Journal of Leukocyte Biology 55, 91–98.CrossRefGoogle ScholarPubMed
Morley, N.J. & Hoole, D. (1995) Ultrastructural studies on the host-parasite interface between Khawia sinensis (Cestoda: Caryophyllidea) and carp (Cyprinus carpio). Diseases of Aquatic Organisms 23, 93–99.CrossRefGoogle Scholar
Nie, P., Hoole, D. & Arme, C. (1996) Proliferation of pronephric lymphocytes of carp, Cyprinus carpio L. induced by extracts of Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda). Journal of Helminthology 70, 127–131.CrossRefGoogle Scholar
Niemczuk, W. (1991). [Histological and histochemical-enzymatical changes in internal organs of the carp infected with tapeworms, Khawia sinensis or Bothriocephalus acheilognathi.] Zeszyty Naukowe Akademii Rolniczej we Wroclawia Wetertgnaria 47, 21–42. [in Polish]Google Scholar
Richards, D.T., Hoole, D., Lewis, J.W., Ewens, E. & Arme, C. (1996) Stimulation of carp Cyprinus carpio lymphocytes in vitro by the blood fluke Sanguinicola inermis (Trematoda: Sanguinicolidae). Diseases of Aquatic Organisms 25, 87–93.CrossRefGoogle Scholar
Rikihisa, Y., Letonja, T., Pratt, N. & Lin, Y.C. (1984) Taenia taeniaeformis: Characterization of larval metabolic products and growth of host gastric cells in vitro. Experimental Parasitology 58, 230–238.CrossRefGoogle ScholarPubMed
Rikihisa, Y., Letonja, T. & Fukaya, T. (1985) Taenia taeniaeformis: inhibition of rat testosterone production by excretory-secretory product of the cultured metacestode. Experimental Parasitology 59, 390–397.CrossRefGoogle ScholarPubMed
Sapozhnikov, G.I. (1973) [A clinical study of Khawia sinensis infection in carp.] in Gagarin, V.G. (Ed.) Problemy obshchei i prikladnoi gel'mintologii. Moscow, USSR; Izdatel'stov “Nauka”. pp.331–336. [in Russian].Google Scholar
Taylor, M.J. & Hoole, D. (1994) Modulation of fish lymphocyte proliferation by extracts and isolated proteinase inhibitors of Ligula intestinalis (Cestoda). Fish and Shellfish Immunology 4, 221–230.CrossRefGoogle Scholar
Touré, Baldè A.T., Sarthou, J-L. & Roussilhon, C. (1995) Acute Plasmodium falciparum infection is associated with increased percentages of apoptotic cells. Immunology Letters 46, 59–62.Google Scholar
Wyllie, A.H. (1988) Apoptosis. ISI Atlas of Science: Immunology 1, 192–196.Google Scholar