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Partial characterization of proteolytic enzymes in different developmental stages of Ostertagia ostertagi

Published online by Cambridge University Press:  05 June 2009

H. de Cock ,
D. P. Knox ,
E. Claerebout  and
D. C. de Graaf
H. de Cock
Affiliation:
Laboratory of Parasitology, Faculty of Veterinary Medicine, University of Gent, Casinoplein 24, B-9000 Gent, Belgium
D. P. Knox
Affiliation:
Moredun Research Institute, 408 Gilmerton Road, Edinburgh, EH17 7JH, UK
E. Claerebout
Affiliation:
Laboratory of Parasitology, Faculty of Veterinary Medicine, University of Gent, Casinoplein 24, B-9000 Gent, Belgium
D. C. de Graaf
Affiliation:
Laboratory of Parasitology, Faculty of Veterinary Medicine, University of Gent, Casinoplein 24, B-9000 Gent, Belgium
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Abstract

Proteolytic enzymes present in extracts of third (L3) and fourth (L4) stage larvae and adults of the cattle nematode Ostertagia ostertagi were defined on the basis of pH optima and proteinase inhibitor sensitivity in spectrophotometric assays using azocasein and elastin-orcein as protein substrates. Evidence that different classes of proteinases are expressed in a stage specific manner was provided by the contrasting pH optima and inhibitor sensitivities shown by the enzymes in the different parasite stages. Stage specificity was confirmed by gelatin-substrate analysis. In addition, proteolytic activity was sought in the excretory/secretory products (ES) of the L4 following simple in vitro culture. Contrasting pH and inhibitor sensitivities as well as gelatin-substrate analysis showed that different proteinases were present in somatic L4 extracts and L4 ES products. The secreted proteinases may be useful targets for serodiagnosis or vaccination.

Keywords

NematodaOstertagia ostertagiproteasesexcretion/secretion productsinhibitorenzymes
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 4 , December 1993 , pp. 271 - 278
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Asch, H. L. & Dresden, M. H. (1979) Acidic thiol proteinase activity of Schistosoma mansoni egg extracts. Journal of Parasitology, 65, 543–549.CrossRefGoogle ScholarPubMed
Barrett, A. J. (1977) Introduction to the history and classification of tissue proteinases. In: Proteinases in Mammalian Cells and Tissues. (editor, Barrett, A. J). pp. 1–52. Elsevier: Amsterdam.Google Scholar
Canals, A. & Gasbarre, L. C. (1989) Procedure for the recovery of excretory-secretory products from molting fourth-stage larvae of Osrertagia osrertagi. Proceedings of the Helminthological Society of Washington, 56, 201–203.Google Scholar
Cox, G. N., Pratt, D., Hageman, R. & Bienvenue, R. J. (1990) Molecular cloning and primary sequence of a cysteine proteinase expressed by adult Haemonchus contortus. Molecular and Biochemical Parasitology, 41, 25–34.CrossRefGoogle Scholar
Dresden, M. H., Rege, A. A. & Murrell, K. D. (1985) Strongyloides ransomi: Proteolytic enzymes from larvae. Experimental Parasirology, 59, 257–263.CrossRefGoogle ScholarPubMed
Emery, D. L. & Wagland, B. M. (1991) Vaccines against gastrointestinal nematode parasites of ruminants. Parasitology Today, 7, 347–349.CrossRefGoogle ScholarPubMed
Hotez, P. J. & Cerami, A. C. (1983) Secretion of a proteolytic anticoagulant by Ancylostoma hookworms. Journal of Experimental Medicine, 157, 1594–1603.CrossRefGoogle ScholarPubMed
Hotez, P. J., Le Trang, N., McKerrow, J. H. & Cerami, A. (1985) Isolation and characterisation of a proteolytic enzyme from the adult hookworm Ancylostoma caninam. Journal of Biological Chemistry, 260, 7343–7348.CrossRefGoogle Scholar
Knox, D. P. & Jones, D. G. (1990) Studies on the presence and the release of proteolytic enzymes (proteinases) in gastrointestinal nematodes of ruminants. International Journal for Parasitology, 20, 243–249.CrossRefGoogle ScholarPubMed
Knox, D. P. & Jones, D. G. (1991) Diagnosis and control of ruminant nematodiasis. In: Parasitic Nematodes—Antigens, Membranes and Genes. (editor, Kennedy, M. W). pp. 170–194, Taylor & Francis: London-New York-Philadelphia.Google Scholar
Knox, D. P. & Kennedy, W. P. (1988) Proteinases released by the parasitic larval stages of Ascaris suum, and their inhibition by antibody. Molecular and Biochemical Parasitology, 28, 207–216.CrossRefGoogle ScholarPubMed
Knox, D. P. & Smith, S. (1991) Proteinases of adult Teladorsargia circumcincta released in vitro. Transactions of the Royal Society for Tropical Medicine and Hygiene, 85, 841.Google Scholar
Knox, D. P., Redmond, D. L. & Jones, D. G. (1993) Characterisation of proteinases in extracts of adult Haemonchus contortus, the ovine abomasal nematode. Parasitology, 106, 395–404.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970) Cleavage of structural proteins during the assemblage of the head of bacteriophage T4. Nature, 227, 680–685.CrossRefGoogle Scholar
Lackey, A., James, E. R., Sakanari, J. A., Resnick, S. D., Brown, M., Bianco, A. E. & McKerrow, J. H. (1989) Extracellular proteases of Onchocerca. Experimental Parasitology, 68, 176–185.CrossRefGoogle ScholarPubMed
Maki, J., Furuhashi, A. & Yanagisawa, T. (1981) The activity of acid proteases hydrolysing haemoglobin in parasitic helminths with special reference to interspecific and intraspecific distribution. Parasitology, 84, 137–147.CrossRefGoogle Scholar
McKerrow, J. H. & Doenhoff, M. J. (1988) Shistosome proteases. Parasitology, 4, 334–340.Google Scholar
McKerrow, J. H., Jones, P., Sage, H. & Pino-Heiss, S. (1985) Proteinases from invasive larvae of the trematode parasite Schistosoma mansoni degrade connective-tissue and basement-membrane macromolecules. Biochemical Journal, 231, 47–51.CrossRefGoogle ScholarPubMed
McKerrow, J. H., Brindley, P., Brown, M., Gam, A. A., Staunton, C. & Neva, F. A. (1990) Strongyloides stercoralis: Identification of a protease that facilitates penetration of skin by the infective larvae. Experimental Parasitology, 70, 134–143.CrossRefGoogle ScholarPubMed
Miller, H. R. P. (1984) The protective mucosal response against gastrointestinal nematodes in ruminants and the laboratory animals. Veterinary Immunopathology, 6, 167–259.CrossRefGoogle ScholarPubMed
Petralanda, I., Yarzabal, L. & Piessens, W. F. (1986) Studies on a filarial antigen with collagenase activity. Molecular and Biochemical Parasitology, 19, 51–59.CrossRefGoogle ScholarPubMed
Rege, A. A. & Dresden, M. H. (1987) Strongyloides spp.: Demonstration and partial characterisation of acidic collagenolytic activity from infective larvae. Experimental Parasitology, 64, 275–280.CrossRefGoogle ScholarPubMed
Robertson, B. D., Bianco, A. E., McKerrow, J. H. & Maizels, R. M. (1989) Toxocara canis: Proteolytic enzymes secreted by the infective larvae in vitro. Experimental Parasitology, 69, 30–36.CrossRefGoogle ScholarPubMed
Smith, W. D. (1988) Mechanisms of immunity to gastrointestinal nematodes of sheep. In: Increasing Small Ruminant Productivity in Semi-arid Areas. (editor, Thompson, F. S.) pp. 275–285.CrossRefGoogle Scholar