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Ultrastructural changes to the tegumental system and gastrodermal cells of adult Fasciola hepatica following treatment in vivo with a commercial preparation of myrrh (Mirazid)

Published online by Cambridge University Press:  20 October 2016

M.M.O. Abdelaal ,
G.P. Brennan ,
A. Abdel-Aziz  and
I. Fairweather
M.M.O. Abdelaal
Affiliation:
Zoology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK
G.P. Brennan
Affiliation:
Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK
A. Abdel-Aziz
Affiliation:
Zoology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
I. Fairweather*
Affiliation:
Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK
*
*Fax: +44-28-90975877 E-mail: i.fairweather@qub.ac.uk
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Abstract

An in vivo study in the laboratory rat model has been carried out to monitor changes to the tegument and gut of adult Fasciola hepatica following treatment with myrrh (‘Mirazid’). Rats infected with the triclabendazole-resistant Dutch isolate were dosed orally with Mirazid at a concentration of 250 mg/kg and flukes recovered 2, 3 and 7 days post-treatment (pt). The flukes were processed for examination by scanning and transmission electron microscopy. A variety of changes to the external surface were observed, culminating in the sloughing of the tegumental syncytium. Internal changes to the syncytium and tegumental cell bodies were more severe and were evident from 2 days pt onwards. Swelling of the basal infolds (leading to flooding of the surface layer) and a decline in secretory body production were the major changes seen. The gastrodermal cells were less severely affected than the tegument, pointing to a trans-tegumental route of uptake for Mirazid by the fluke. Some loss of muscle fibres in the main somatic muscle layers was observed, which may be correlated with the decline in movement of flukes seen at recovery.

Type
Research Papers
Information
Journal of Helminthology , Volume 91 , Issue 6 , November 2017 , pp. 672 - 685
Copyright
Copyright © Cambridge University Press 2016 

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