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The use of fluorescence lifetime technology in benign and malignant thyroid tissues

Published online by Cambridge University Press:  10 July 2019

G Nakache ,
G Yahav ,
G H Siloni ,
I Barshack ,
E Alon ,
M Wolf  and
D Fixler
G Nakache*
Affiliation:
Department of Otolaryngology Head and Neck Surgery, Rabin Medical Center, Petach Tikva, Israel
G Yahav
Affiliation:
Faculty of Engineering, Bar Ilan University, Raman Gan, Israel
G H Siloni
Affiliation:
Sheba Tissue Bank, Sheba Medical Center, Ramat Gan, Israel
I Barshack
Affiliation:
Department of Pathology, Sheba Medical Center, Ramat Gan, Israel
E Alon
Affiliation:
Department of Otolaryngology Head and Neck Surgery, Sheba Medical Center, Ramat Gan, Israel
M Wolf
Affiliation:
Department of Otolaryngology Head and Neck Surgery, Sheba Medical Center, Ramat Gan, Israel
D Fixler
Affiliation:
Faculty of Engineering, Bar Ilan University, Raman Gan, Israel
*
Author for correspondence: Dr Gabriel Nakache, Department of Otolaryngology Head and Neck Surgery, Rabin Medical Center, Petach Tikva, Israel E-mail: nakachega@gmail.com
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Abstract

Objective

To explore the use of fluorescence lifetime imaging microscopy in thyroid tissues, and to investigate how different thyroid lesions affect fluorescence lifetime.

Method

Fluorescence lifetime measurements were taken of fresh frozen thyroid surgical specimens stained with fluorescein isothiocyanate tagged anti-thyroglobulin monoclonal antibodies.

Results

The mean fluorescence lifetime measurements in 12 patients – 3 with multinodular goitre, 4 with follicular adenoma, 4 with papillary thyroid carcinoma and 1 with follicular carcinoma – were 3.16 ns (range, 2.66–3.52 ns), 3.75 ns (range, 2.99–4.57 ns), 2.97 ns (range, 2.57–3.21 ns) and 3.61 ns, respectively. The fluorescence lifetime of follicular adenoma patients was higher than that of papillary thyroid carcinoma patients by 26 per cent (p = 0.058). The fluorescence lifetime in the follicular carcinoma patient was similar to the follicular adenoma group, but higher than in the papillary thyroid carcinoma group by 22 per cent (p = 0.01).

Conclusion

Fluorescence lifetime measurements varied in different thyroid pathologies, possibly because of tissue-scale structural influences.

Keywords

Thyroid NoduleFluorescence Microscopy
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 133 , Issue 8 , August 2019 , pp. 696 - 699
Copyright
Copyright © JLO (1984) Limited, 2019 

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Footnotes

Dr G Nakache takes responsibility for the integrity of the content of the paper

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