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Indentation micromechanics of three-dimensional fibrin/collagen biomaterial scaffolds

Published online by Cambridge University Press:  01 August 2006

R.G. Mooney ,
C.A. Costales ,
E.G. Freeman ,
J.M. Curtin ,
A.A. Corrin ,
J.T. Lee ,
S. Reynolds ,
B. Tawil  and
M.C. Shaw
R.G. Mooney
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
C.A. Costales
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
E.G. Freeman
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
J.M. Curtin
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
A.A. Corrin
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
J.T. Lee
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
S. Reynolds
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
B. Tawil
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
M.C. Shaw*
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
*
a) Address all correspondence to this author. e-mail: mcshaw@clunet.edu
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Abstract

The underlying relationships between the microstructure and time-dependent mechanical properties of hydrated fibrin, collagen, and fibrin/collagen composite materials have been explored using an adaptation of the classical rigid, cylindrical, flat punch loaded normally to a planar specimen surface. A suite of quasi-static elastic and viscoelastic indentation experiments have been conducted with uniformly mixed fibrin, collagen, and fibrin/collagen composites, in addition to macrolayered collagen materials. Coupled with insights obtained from optical and confocal fluorescence microscopy, a simple micromechanics model has been developed for the effect of local microstructural variables on the macroscopic mechanical stiffness. These results demonstrate the efficacy of this technique to efficiently and reproducibly probe hydrated engineered tissue replacement materials for local variations in viscoelastic material behavior without the need for extensive specimen preparation or grips, as well as being suitable for performing directly comparable measurements with explants of human skin.

Keywords

BiomedicalElastic propertiesMicrostructure
Type
Articles
Information
Journal of Materials Research , Volume 21 , Issue 8 , August 2006 , pp. 2023 - 2034
Copyright
Copyright © Materials Research Society 2006

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