Hostname: page-component-848d4c4894-75dct Total loading time: 0 Render date: 2024-06-07T07:47:11.423Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic variation in relation to substratum preferences of Hypogymnia physodes

Published online by Cambridge University Press:  06 August 2009

Jan-Eric MATTSSON ,
Anne-Charlotte HANSSON  and
Louise LINDBLOM
Jan-Eric MATTSSON
Affiliation:
School of Life Sciences, Södertörn University, SE-141 89 Huddinge, Sweden. Email: jan-eric.mattsson@sh.se
Anne-Charlotte HANSSON
Affiliation:
School of Life Sciences, Södertörn University, SE-141 89 Huddinge, Sweden.
Louise LINDBLOM
Affiliation:
Department of Biology & Museum of Natural History, University of Bergen, P.O. Box 7800, NO-5020 Bergen, Norway.
Get access Rights & Permissions [Opens in a new window]

Abstract

Genetic variability and its relationship to substratum preferences within and among populations of the sorediate foliose lichen Hypogymnia physodes was investigated using sequence variation in the complete nrDNA internal transcribed spacer (ITS) region. A few samples of the putatively closely related, sorediate, H. tubulosa were also included. Samples were collected from each tree species in study sites in Estonia, Finland, and Sweden. In total, DNA sequences from 104 individuals of H. physodes and 16 of H. tubulosa were obtained. A group 1 intron situated at the end of the small subunit (SSU) of the nrDNA was detected in both species. Within-species variability was observed in both species: fifteen haplotypes were found for H. physodes and seven for H. tubulosa for the combined alignment of the intron and the ITS. Possible recombination within the total gene fragment was detected and hence the different regions (intron, ITS1, 5.8S, ITS2) were analysed separately. They show a different degree of variability both between each other and between the species. The number of haplotypes of H. physodes in the four regions are 5, 5, 1, and 5 and for H. tubulosa 5, 2, 1 and 2, respectively. A statistical parsimony estimation resulted in two unconnected networks; one containing all the samples of H. physodes and one containing all H. tubulosa samples. It was not possible to show different potentials of the different haplotypes for establishment on different substrata as the network of H. physodes indicates recombination within the ITS region which may be frequent enough to make this primarily clonally reproducing species to behave like a sexual species.

Keywords

ascomycetesdispersalhaplotype networksHypogymnia tubulosalichensParmeliaceaepopulationssystematics
Type
Research Article
Information
The Lichenologist , Volume 41 , Issue 5 , September 2009 , pp. 547 - 555
Copyright
Copyright © British Lichen Society 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Buschbom, J. & Mueller, G. (2004) Resolving evolutionary relationships in the lichen-forming genus Porpidia and related allies (Porpidiaceae, Ascomycota). Molecular Phylogenetics and Evolution 32: 66–82.CrossRefGoogle ScholarPubMed
Cassie, D. M. & Piercey-Normore, M. D. (2008) Dispersal in a sterile lichen-forming fungus, Thamnolia subuliformis (Ascomycotina: Icmadophilaceae). Botany/Botanique: 86: 751–762.CrossRefGoogle Scholar
Clement, M., Posada, D. & Crandall, K. A. (2000) TCS: a computer program to estimate gene genealogies. Molecular Ecology 9: 1657–1659.CrossRefGoogle ScholarPubMed
DuRietz, G. E. (1945) Om fattigbark- och rikbarksamhällen. Svensk Botanisk Tidsskrift 39: 147–150.Google Scholar
Excoffier, L., Laval, G. & Schneider, S. (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1: 47–50.Google Scholar
Fahselt, D. & Hageman, C. (1990) Enzyme electromorph variation in the lichen family Umbilicariaceae – within-stand polymorphism in umbilicate lichens of eastern Canada. Canadian Journal of Botany 68: 2636–2643.Google Scholar
Gardes, M. & Bruns, T. D. (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118.CrossRefGoogle Scholar
Gargas, A., DePriest, P. T., Grube, M. & Tehler, A. (1995) Multiple origins of lichen symbioses in fungi suggested by SSU rDNA phylogeny. Science 268: 1492–1495.CrossRefGoogle ScholarPubMed
Hudson, R. R. & Kaplan, N. L. (1985) Statistical properties in the number of recombination events in the history of a sample of DNA sequences. Genetics 111: 147–164.CrossRefGoogle ScholarPubMed
Lättman, H., Brand, A., Hedlund, J., Krikorev, M., Olsson, N., Robeck, A., Rönnmark, F. & Mattsson, J.-E. (2009) Generation length on Cliostomum corrugatum (Ach.). Lichenologist 41: 557–559.CrossRefGoogle Scholar
Moberg, R. & Holmåsen, I. (1990) Lavar, en fälthandbok. 3rd ed. Stockholm: Interpublishing.Google Scholar
Printzen, C. & Ekman, S. (2002) Genetic variability and its geographical distribution in the widely disjunct Cavernularia hultenii. Lichenologist 34: 101–111.CrossRefGoogle Scholar
Printzen, C., Ekman, S. & Tønsberg, T. (2003) Phylogeography of Cavernularia hultenii: evidence of slow genetic drift in a widely disjunct lichen. Molecular Ecology 12: 1473–1486.CrossRefGoogle Scholar
Raymond, M. & Rousset, F. (1995) An exact test for population differentiation. Evolution 49: 1280–1283.CrossRefGoogle ScholarPubMed
Rozas, J. & Rozas, R. (1999) DnaSP version 3: an integrated program for molecular population genetics and molecular evolution analysis. Bioinformatics 15: 174–175.CrossRefGoogle ScholarPubMed
Templeton, A. R., Crandall, K. A. & Sing, C. F. (1992) A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics 132: 619–633.CrossRefGoogle ScholarPubMed
Vinter, T. (2006) Habitat associations and fitness in Daphne mezereum. M. Sc. thesis, Södertörn University College.Google Scholar
White, T. J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications (Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J., eds): 315–322. San Diego: Academic Press.Google Scholar