Chameleon: Next Generation Sample Preparation for CryoEM based on Spotiton

Michele C. Darrow; John P. Moore; R. John Walker; Klaus Doering; Russell S. King

doi:10.1017/S1431927619005701

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References

[1]Razinkov, I et al. , Journal of Structural Biology 195 (2016), p. 190.Google Scholar

[2]Dandey, V et al. , Journal of Structural Biology 202 (2018), p. 161.Google Scholar

[3]Wei, H et al. , Journal of Structural Biology 202 (2018), p. 170.Google Scholar

[4]Glaeser, R et al. , Biophysical Journal 110 (2016), p. 749.Google Scholar

[5]Glaeser, R, Current Opinion in Colloid & Interface Science 34 (2018), p. 1.Google Scholar

[6]Noble, A et al. , Nature Methods 15 (2018), p. 793.Google Scholar

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Chameleon: Next Generation Sample Preparation for CryoEM based on Spotiton

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