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Correlative light and electron microscopy technique using commercially available reagents to facilitate immunolocalization via epi-fluorescence and TEM

Published online by Cambridge University Press:  05 August 2019

Leslie Cummins ,
Vincent Tu ,
Louis M. Weiss  and
Frank P. Macaluso
Leslie Cummins*
Affiliation:
Department of Anatomy and Structural Biology/Albert Einstein College of Medicine, Bronx, NY, USA. Analytical Imaging Facility, Albert Einstein College of Medicine, Bronx, NY, USA.
Vincent Tu
Affiliation:
Albert Einstein College of Medicine, Department of Pathology, Bronx, NY, USA
Louis M. Weiss
Affiliation:
Albert Einstein College of Medicine, Department of Pathology, Bronx, NY, USA Albert Einstein College of Medicine, Department of Medicine, Bronx, NY, USA
Frank P. Macaluso
Affiliation:
Department of Anatomy and Structural Biology/Albert Einstein College of Medicine, Bronx, NY, USA. Analytical Imaging Facility, Albert Einstein College of Medicine, Bronx, NY, USA. Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, USA
*
*Corresponding author: Leslie.Gunther@einstein.yu.edu

Abstract

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Type
Multi-Modal, Large-Scale and 3D Correlative Microscopy
Information
Microscopy and Microanalysis , Volume 25 , Supplement S2 , August 2019 , pp. 1054 - 1055
Copyright
Copyright © Microscopy Society of America 2019 

References

[1]Macaluso, Frank P. et al. “Clem Methods for Studying Primary Cilia.” Methods in Molecular Biology. Vol. 1454. (Humana Press Inc., New York, NY) 2016. 193–202.Google Scholar
[2]“Toxoplasma gondii: The Model Apicomplexan. Perspectives and Methods”, ed. Weiss, L.M., Kim, K., (Academic Press, Cambridge, MA) 2007.Google Scholar
[3]Bozzola, JJ. Electron Microscopy. 3rd ed. London, UK: Jones and Bartlett; 2012.Google Scholar
[4]All imaging was conducted in the Analytical Imaging Facility (AIF) (funded by NCI Cancer Grant P30CA013330). TEM Imaging was conducted on a JEOL 1400Plus funded by SIG (1S10OD016214-01A1). The authors would also like to thank Dr. Vera DesMarais for help in editing this manuscript and Xheni Nishku for help with the figures.Google Scholar