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Enhanced Esi-Detection of Metal Using the Core-Edges at Energy-Loss Below 100 Ev

Published online by Cambridge University Press:  02 July 2020

H. Troester ,
A. Haking ,
K. Richter ,
W. Probst  and
M. F. Trendelenburg
H. Troester
Affiliation:
Biomedical Structure Analysis (DKFZ, 0195), German Cancer Research Centre, INF 280, D-69120Heidelberg, Germany
A. Haking
Affiliation:
Biomedical Structure Analysis (DKFZ, 0195), German Cancer Research Centre, INF 280, D-69120Heidelberg, Germany
K. Richter
Affiliation:
Biomedical Structure Analysis (DKFZ, 0195), German Cancer Research Centre, INF 280, D-69120Heidelberg, Germany
W. Probst
Affiliation:
LEO Electron Microscopy GmbH, D-73446Oberkochen, Germany
M. F. Trendelenburg
Affiliation:
Biomedical Structure Analysis (DKFZ, 0195), German Cancer Research Centre, INF 280, D-69120Heidelberg, Germany
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Extract

ESI (electron spectroscopic imaging)-detection methods are mainly based on the core edges above 100 eV (see, as an example, Ref. 1). However, it would be of major interest to use the high intensity values in the low-loss region below 100 eV. There are at least two elements of general interest for biological applications showing core edges in this region: Au (O2,3 ) and Fe (M 2,3 )• Since colloidal gold and ferritin are used to label targets in biological samples at high spatial resolution we started our study aiming at iron-detection (iron-maximum at 62 eV comparable to gold-maximum at 65 eV).

Ferritin (Ref. 2) contains about 200 to 2000 iron atoms in a densely packed core representing an ideal prerequisite for ESI-detection. Furthermore, ferritin used to be a common marker in conventional transmission electron microscopy in the past. The contrast produced by ferritin in a conventional transmission electron microscope (CTEM) is not specific for iron but only for a dense region in the specimen.

Type
Analytical Electron Microscopy
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 979 - 980
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Leapman, R.D. and Andrews, S.B., J. Microscopy 165 (2) (1992) 225–238.10.1111/j.1365-2818.1992.tb01482.xCrossRefGoogle Scholar
2.Massover, W.H., Micron 24(4) (1993) 389–437.10.1016/0968-4328(93)90005-LCrossRefGoogle Scholar
3. This study was supported by a grant from the German Ministry of Science and Education (BMBF, Bonn, Germany, Grant No. 0310944).Google Scholar