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Investigating the Ultrastructural Effects of F-BAR Proteins on Neuritogenesis by CLEM and Cryo-ET

Published online by Cambridge University Press:  22 July 2022

Joseph Y. Kim ,
Lauren A. English ,
Tanner Tenpas ,
Jie E. Yang ,
Erik W. Dent  and
Elizabeth R. Wright
Joseph Y. Kim
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, Madison, WI, United States Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States
Lauren A. English
Affiliation:
Neuroscience Training Program, University of Wisconsin-Madison, Madison, WI, United States
Tanner Tenpas
Affiliation:
Department of Neuroscience, University of Wisconsin-Madison, Madison, WI, United States
Jie E. Yang
Affiliation:
Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States
Erik W. Dent
Affiliation:
Department of Neuroscience, University of Wisconsin-Madison, Madison, WI, United States
Elizabeth R. Wright*
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Morgridge Institute for Research, University of Wisconsin-Madison, Madison, WI, United States
*
*Corresponding author: erwright2@wisc.edu

Abstract

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Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Information
Microscopy and Microanalysis , Volume 28 , Supplement S1 , August 2022 , pp. 1124 - 1127
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Copyright
Copyright © Microscopy Society of America 2022

References

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This work was supported in part by the University of Wisconsin, Madison, the Department of Biochemistry at the University of Wisconsin, Madison, and public health service grants R01 GM114561 and U24 GM139168 to E.R.W. and R01 NS115400 to E.W.D. from the NIH. We are grateful for the use of facilities and instrumentation at the Cryo-EM Research Center in the Department of Biochemistry at the University of Wisconsin, Madison.Google Scholar