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Montage Cryo-Electron Tomography: a High Throughput and Flexible Data Collection Scheme to Explore In-situ Cellular Landscapes while Preserving High-Resolution Data

Published online by Cambridge University Press:  22 July 2022

Jae Yang ,
Matt Larson ,
Bryan Sibert  and
Elizabeth Wright
Jae Yang
Affiliation:
Department of Biochemistry, University of Wisconsin, Madison, WI, USA Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA
Matt Larson
Affiliation:
Department of Biochemistry, University of Wisconsin, Madison, WI, USA Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA
Bryan Sibert
Affiliation:
Department of Biochemistry, University of Wisconsin, Madison, WI, USA Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA
Elizabeth Wright*
Affiliation:
Department of Biochemistry, University of Wisconsin, Madison, WI, USA Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA Morgridge Institute for Research, Madison, WI, USA
*
*Corresponding author: erwright2@wisc.edu

Abstract

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Type
Technical Advances in Cryo-EM
Information
Microscopy and Microanalysis , Volume 28 , Supplement S1 , August 2022 , pp. 1222 - 1224
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Copyright
Copyright © Microscopy Society of America 2022

References

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This work was supported in part by the University of Wisconsin, Madison, the Department of Biochemistry at the University of Wisconsin, Madison, and public health service grants R01 GM114561 and U24 GM139168 to E.R.W. from the NIH. We are grateful for the use of facilities and instrumentation at the Cryo-EM Research Center in the Department of Biochemistry at the University of Wisconsin, Madison.Google Scholar