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A New Attractive Approach of Three- Dimensional(3D) Reconstruction of the Relationships Between Hormone Secreating Cells and its Microvessel Network Patterns in Rat Pituitary Gland by Confocal Laser Scanning Miroscopy(CLSM) With Combined Fluorescent and Non-Fluorescent Probes.

Published online by Cambridge University Press:  02 July 2020

J. Itoh ,
I. Takumi ,
N. Sanno ,
K. Kawai ,
A. Serizawa ,
A. Teramoto  and
R.Y. Osamura
J. Itoh
Affiliation:
Lab for Struct/Funct Res
I. Takumi
Affiliation:
Dept of Neurosurg. Nippon Med Sch Bunkyo-kuTokyo113, Japan
N. Sanno
Affiliation:
Dept of Pathol
K. Kawai
Affiliation:
Div of Diag Pathol Tokai Univ Sch of MedIseharaKanagawa259-11, Japan
A. Serizawa
Affiliation:
Div of Diag Pathol Tokai Univ Sch of MedIseharaKanagawa259-11, Japan
A. Teramoto
Affiliation:
Dept of Neurosurg. Nippon Med Sch Bunkyo-kuTokyo113, Japan
R.Y. Osamura
Affiliation:
Dept of Pathol
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Extract

There have been considerable interests in the relationship between hormone secreting endocrine cells and microvessel network patterns in human pituitary gland. However, its 3D microcirculatory networks have not been studied extensively. Therefore, this study was designed to visualize hormone secreting endcrine cells and microvessels in 3D by CLSM and to reveal relationship between hormone secretion and microvessel networks in the rat pituitary gland.

CLSM has been an exciting new apparatus because of its more powerful resolution than conventional light microscopy and the high-performance on 3D optical sectioning. The images taken with CLSM can be digitized and made susceptible to image analysis manipulation and computer-assisted 3D reconstructions.

The female normal adult Wistar rats were used in this study. To demonstrate microvessels, fluorescent probe( 30 mg/ml)-conjugated gelatin solution was injected to left ventricle of the heart After complete perfusion, the pituitary glands were resected and immediately fixed in cold graded PFA(0.5- 8 %) containing 15 % picric acid solutions (4 °C. GH, ACTH cells or S100 protein positive fcfliculostellate (FS) cells were demonstrated by indirect TRTTC-labeled or HRP labeled antibody methods.

Type
Cytochemistry, Histochemistry, Immunocytochemistry, and in Situ Hybridization
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 165 - 166
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Itoh, J., et al.,(1992), J. Histochem. Cytochem., 40:955–96710.1177/40.7.1607644CrossRefGoogle Scholar
2.Itoh, J.,et al.,MRT (in press)Google Scholar