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Artifacts from Rapid Microwave Processing of Trematode Tissues (Ascocotyle pachycystis and leighi)

Published online by Cambridge University Press:  14 March 2018

Mark H. Armitage
Mark H. Armitage*
Affiliation:
Liberty University, Thousand Oaks, CA
*
mharmitage@liberty.edu

Extract

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The use of microwave energy to assist in the processing of biological tissues for microscopy has generated significant interest in recent years. Microwave (MW) processing has been used to prepare tissues for light microscopy (Carranza et al. 1990 [using parasite tissues]; van Dorp et al. 1995; Davis et al. 1997; Izumi et al 2000; and Rohr et al. 2001), as well as for electron microscopy (Kasa et al. 1982; Hopwood et al. 1984; Leong et al. 1985; Kang et al, 1991 [using parasite tissues]; Heumann 1992; Wagenaar et al. 1993; Login and Dvorak 1993; Giberson and Demaree 1995; Madden and Miriam 1997; Giberson et al. 1997; Morin et al. 1997; Petrali and Mills 1999; Massa and Arana-Chavez 2000; Hernandez and Guillen 2000 [using parasite tissues]; Demaree 2001; Giberson 2001).

Most reports, particularly those published by manufacturers of microwave ovens, have shown positive results regarding tissue ultrastructure, however discussion continues on possible mechanisms of preservation by the use of MW technology.

Type
Research Article
Information
Microscopy Today , Volume 12 , Issue 5 , September 2004 , pp. 32 - 35
Copyright
Copyright © Microscopy Society of America 2004

References

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