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Effect of chemical substitution on the surface charge of the photosynthetic Reaction Center from Rhodobacter sphaeroides: an in-silico investigation.

Published online by Cambridge University Press:  25 June 2020

Gabriella Buscemi ,
Francesco Milano ,
Danilo Vona ,
Gianluca M. Farinola  and
Massimo Trotta Open the ORCID record for Massimo Trotta [Opens in a new window]
Gabriella Buscemi
Affiliation:
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona 4 70126, BariItaly IPCF-CNR Istituto per i processi Chimico-Fisici, Consiglio Nazionale delle Ricerche, Via Orabona 4, 70126, Bari, Italy
Francesco Milano
Affiliation:
IPCF-CNR Istituto per i processi Chimico-Fisici, Consiglio Nazionale delle Ricerche, Via Orabona 4, 70126, Bari, Italy
Danilo Vona
Affiliation:
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona 4 70126, BariItaly
Gianluca M. Farinola
Affiliation:
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona 4 70126, BariItaly
Massimo Trotta
Affiliation:
IPCF-CNR Istituto per i processi Chimico-Fisici, Consiglio Nazionale delle Ricerche, Via Orabona 4, 70126, Bari, Italy
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Abstract

The Reaction Centers (RCs) proteins are membrane proteins representing the key component so flight energy transduction in photosynthetic organisms. Upon photon absorption, these photoenzymes produce a long lasting intra protein hole electron couples whose charges are separated by 3 nanometers. The dipoles formed within the RCs can be effectively employed as transducing cores of several biological-organic hybrid devices whose design can accomplish photocurrents generation or act as phototransistor. To widen the application of the RCs to as many substrate as possible one valuable strategy is the bioconjugation of the protein with specific molecules ad-hoc selected to improve enzymatic performance and/or integration in proper scaffolding. In the present manuscript, we investigate the changes of the isoelectric point of the RC from the carotenoidless strain of the photosynthetic bacterium Rhodobacter sphaeroides R26 by inducing “in silico” mutations to predict on the role of the aminoacids involved in the bioconjugation.

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MRS Advances , Volume 5 , Issue 45: Soft Materials and Biomaterials , 2020 , pp. 2309 - 2316
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Copyright © Materials Research Society 2020

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References

Rasmussen, M., Minteer, S. D., J. Electrochem. Soc. 2011, 161, H647-H655.CrossRefGoogle Scholar
Milano, F., Tangorra, R.R., Hassan Omar, O., Ragni, R., Operamolla, A., Agostiano, A., Farinola, G.M., and Trotta, M., Angew. Chem. Int. Ed. 2012, 5, 11019-11023.Google Scholar
Dutta, P. K., Lin, S., Loskutov, A., Levenberg, S., Jun, D., Saer, R., Beatty, J. T., Liu, Y., Yan, H., Woodbury, N. W., J. Am. Chem. Soc. 2014, 136, 4599–4604.CrossRefGoogle Scholar
Milano, F., Punzi, A., Ragni, R., Trotta, M., Farinola, G. M., Adv. Funct. Mater. 2019, 29, 1805521.CrossRefGoogle Scholar
Milano, F., Ciriaco, F., Trotta, M., Chirizzi, D., De Leo, V., Agostiano, A., Valli, L., Giotta, L., Guascito, M.R., Electrochim. Acta 2019, 293, 105-115.CrossRefGoogle Scholar
Liu, J., Mantell, J., Jones, M.R., ACS Nano 2020, 14, 4536–4549.CrossRefGoogle Scholar
Milano, F., Tangorra, R.R., Agostiano, A., Giotta, L., De Leo, V., Ciriaco, F., Trotta, M., MRS Adv. 2018, 3, 1497-1507.CrossRefGoogle Scholar
Allen, J.P., Feher, G., Yeates, T.O., Komiya, H., Rees, D.C., Proc. Natl. Acad. Sci, 1988, 85, 8487-8491.CrossRefGoogle Scholar
Koepke, J., Krammer, E.M., Klingen, A.R., Sebban, P., Ullmann, G.M., Fritzsch, G., J Mol Biol. 2007, 371, 396-409.CrossRefGoogle Scholar
Tangorra, R. R.; Antonucci, A.; Milano, F.; la Gatta, S.; Farinola, G. M.; Agostiano, A.; Ragni, R.; Trotta, M. in Handbook of Photosynthesis, Third Edition CRC Press, 2016, 201-220.Google Scholar
Ragni, R., Leone, G., La Gatta, S., Rizzo, G., Lo Presti, M., De Leo, V., Farinola, G.M., MRS Adv. 2019, 4, 1143-1148.CrossRefGoogle Scholar
Feher, G., Allen, J.P., Okamura, M.Y., Rees, D.C., Nature 1989, 339, 111–116.CrossRefGoogle Scholar
Trotta, M., Milano, F., Nagy, L., Agostiano, A., Mater. Science and Eng. C, 2002, 22, 263-267.CrossRefGoogle Scholar
Lo Presti, M., Vona, D., Leone, G., Rizzo, G., Ragni, R., Cicco, S. R., Milano, F., Palumbo, F., Trotta, M., Farinola, G. M., MRS Adv. 2019, 4, 1741-1748.CrossRefGoogle Scholar
Hajdu, K., Gergely, C., Martin, M., Cloitre, T., Zimányi, L., Tenger, K., Khoroshyy, P., Palestino, G., Agarwal, V., Hernádi, K., Németh, Z., Nagy, L., Langmuir, 2012, 28, 11866-11873.CrossRefGoogle Scholar
Lo Presti, M., Giangregorio, M.M., Ragni, R., Giotta, L., Guascito, M.R., Comparelli, R., Fanizza, E., Tangorra, R.R., Agostiano, A., Losurdo, M., Farinola, G.M., Milano, F., Trotta, M., Adv. Electron. Mater. 2020, 2000140.CrossRefGoogle Scholar
Di Lauro, M., La Gatta, S., Bortolotti, C.A., Beni, V., Parkula, V., Drakopoulou, S, Giordani, M., Berto, M., Milano, F., Cramer, T., Murgia, M., Agostiano, A., Farinola, G.M., Trotta, M., Biscarini, F., Adv. Electron. Mater. 2020, 6, 1900888.CrossRefGoogle Scholar
Di Lauro, M., Buscemi, G., Bianchi, M., De Salvo, A., Berto, M., Carli, S., Trotta, M., MRS Adv. 2020, 5, 985-990.CrossRefGoogle Scholar
Takahashi, Y., Utsumi, K., Yamamoto, Y., Hatano, A., Satoh, K., Plant and Cell Physiology, 1996, 37, 161–168.CrossRefGoogle Scholar
Espiritu, E., Chamberlain, K.D., Williams, J.C., Allen, J.P.. Photosynthesis Research, 2020, 143, 129-141.CrossRefGoogle Scholar
La Gatta, S., Hassan Omar, O., Agostiano, A., Milano, F., Tangorra, R.R., Operamolla, A., Ragni, R., MRS Advances, 2016, 1, 495-500.CrossRefGoogle Scholar
Heifler, O., Carmeli, C., Carmeli, I., Langmuir, 2020, 36, 4556-4562.CrossRefGoogle Scholar
Altamura, E., Milano, F., Tangorra, R.R., Trotta, M., Hassan Omar, O., Stano, P., Mavelli, F., Proc. Natl. Acad. Sci. USA, 2017, 114, 3837-3842.CrossRefGoogle Scholar
Audain, E., Ramos, Y., Hermjakob, H., Flower, D.R., Perez-Riverol, Y. Bioinformatics, 2016, 32, 821–827.CrossRefGoogle Scholar
Kozlowski, L.P., Biol. Direct 2016, 11, 55.CrossRefGoogle Scholar
Williams, J.C., Steiner, L.A., Ogden, R.C., Simon, M.I., Feher, G., Proc. Natl. Acad. Sci. USA, 1983, 80, 6505-6509.CrossRefGoogle Scholar
Williams, J.C., Steiner, L.A., Ogden, R.C., Simon, M.I., Feher, G., Proc. Natl. Acad. Sci. USA, 1984, 80, 7303-7307.CrossRefGoogle Scholar
Williams, J.C., Steiner, L.A., Feher, G., Proteins, 1986, 1, 312-325.CrossRefGoogle Scholar
Prince, R.C., Cogdell, R.J., Crofts, A.R., BBA - Bioenergetics, 1974, 347, 1-13.CrossRefGoogle Scholar