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Microstructure And Mechanical Properties Of Skeletal Bone In Gene-Mutated Stöpseldtl28d And Wild-Type Zebrafish (Danio Rerio)

Published online by Cambridge University Press:  17 March 2011

Yang Zhang ,
Fu-Zhai Cui ,
Xiu-Mei Wang ,
Qang Cai  and
Qing-Ling Feng
Yang Zhang
Affiliation:
Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
Fu-Zhai Cui
Affiliation:
Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
Xiu-Mei Wang
Affiliation:
Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
Qang Cai
Affiliation:
Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
Qing-Ling Feng
Affiliation:
Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
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Abstract

Zebrafish (Danio rerio), the only vertebrate organism now amenable to large scale of “saturation genetic screens”, provides a single opportunity to investigate the complex gene mechanisms for normally vertebrate-specific processes. Studies of bone formation and bone diseases utilizing zebrafish system are therefore of interest. In the present study, microstructure and mechanical properties of skeletal bone in gene-mutated Stöpsel dtl28d (stp/stp) and wild-type zebrafish were investigated using transmission electron microscopy (TEM) and an atomic force microscopy (AFM)-based nanoindenter. Abnormal collagen matrix and minerals were observed in stp/stp bone comparing to the wild-type control. Nanoindentation measurement disclosed that there was a significant increase of elastic modulus in stp/stp bone compared to that of the control. AFM examinations of the residual indenter impressions indicate that the bone of zebrafish becomes more brittle after stp gene-mutation. This observation of the alteration of bone collagen, minerals and mechanical behavior by gene-mutation in zebrafish system is of scientific and clinical importance in many fields, such as bone fracture and brittleness in human heritable disorders and materials fabrication of bone tissue engineering via gene control.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 711: Symposia FF/GG/HH – Advanced Biomaterials--Characterization, Tissue Engineering and Complexity , 2001 , FF3.9.1
Copyright
Copyright © Materials Research Society 2002

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