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Intraspecific genetic variation of Stagonospora avenae and its differentiation from S. nodorum

Published online by Cambridge University Press:  01 May 1998

P. P. UENG ,
K. SUBRAMANIAM ,
W. CHEN ,
E. ARSENIUK ,
LIXIN WANG ,
A. M. CHEUNG ,
G. M. HOFFMANN  and
G. C. BERGSTROM
P. P. UENG
Affiliation:
Plant Molecular Biology Laboratory, USDA-ARS, BARC-West, Beltsville, MD 20705, U.S.A.
K. SUBRAMANIAM
Affiliation:
Plant Molecular Biology Laboratory, USDA-ARS, BARC-West, Beltsville, MD 20705, U.S.A.
W. CHEN
Affiliation:
Illinois Natural History Survey, University of Illinois, Champaign, IL 61820, U.S.A.
E. ARSENIUK
Affiliation:
Department of Plant Pathology, Plant Breeding and Acclimatization Institute, Radzików, Poland
LIXIN WANG
Affiliation:
Plant Molecular Biology Laboratory, USDA-ARS, BARC-West, Beltsville, MD 20705, U.S.A. Present address: Beijing Plant Cell Bioengineering Laboratory, Beijing, China.
A. M. CHEUNG
Affiliation:
Cellular and Molecular Genetics Program, University of Maryland, College Park, MD 20742, U.S.A.
G. M. HOFFMANN
Affiliation:
Technische Universität München, Lehrstuhl für Phytopathologie, Germany
G. C. BERGSTROM
Affiliation:
Department of Plant Pathology, Cornell University, Ithaca, NY 14853, U.S.A.
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Abstract

To identify Stagonospora avenae genetically, 53 isolates from Europe and North America were assessed by RFLP analysis and internal transcribed spacer (ITS) sequence polymorphisms in comparison with isolates of S. nodorum, S. arenaria and Septoria tritici. Our results also provided insight on possible phylogenetic divergence within the species currently identified by characteristic conidial morphology as S. avenae. Isolates from Europe, Canada, Minnesota and North Dakota showed little genetic variation. In RFLP and ITS sequence analyses, S. avenae isolates from New York and ATCC 12277 (origin unknown) had a closer relationship with barley-biotype S. nodorum, and S. avenae f. sp. triticea isolates of ATCC 26370 and ATCC 26377 from Minnesota were closely associated with wheat-biotype S. nodorum. Enzyme restrictions of ITS PCR-amplified products could facilitate the identification of Stagonospora on cereals.

Type
Research Article
Information
Mycological Research , Volume 102 , Issue 5 , May 1998 , pp. 607 - 614
Copyright
The British Mycological Society 1998

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