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Analysis of the beta-tubulin codon 200 genotype distribution in a benzimidazole-susceptible and -resistant cyathostome population

Published online by Cambridge University Press:  09 October 2003

M. PAPE ,
J. POSEDI ,
K. FAILING ,
T. SCHNIEDER  and
G. von SAMSON-HIMMELSTJERNA
M. PAPE
Affiliation:
Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany
J. POSEDI
Affiliation:
Institute of Microbiology and Parasitology, Veterinary Faculty of Ljubljana, University of Ljubljana, Slovenia
K. FAILING
Affiliation:
Department for Biomathematics and Data Processing, Faculty of Veterinary Medicine, Justus-Liebig-University, Frankfurter Str. 95, D-35392 Giessen, Germany
T. SCHNIEDER
Affiliation:
Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany
G. von SAMSON-HIMMELSTJERNA
Affiliation:
Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany
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Abstract

To study the prevalence of the polymorphism in position 200 of the beta-tubulin gene in the mechanism of benzimidazole (BZ) resistance in cyathostomes of horses, an allele-specific PCR was used to detect the genotype of individuals of BZ-susceptible and BZ-resistant populations. The molecular analysis of 100 adults recovered from an anthelmintic-naïve horse revealed 80% homozygous TTC/TTC individuals, 17% heterozygous TTC/TAC and 3% homozygous TAC/TAC. A naturally infected horse was treated with increasing fenbendazole (FBZ) dosages to select a BZ-resistant population of cyathostomes. The PCR based analysis of 3rd-stage larvae (L3) during the experiment revealed a decrease of the homozygous TTC/TTC genotype and an increase in heterozygous TTC/TAC and homozygous TAC/TAC individuals. After treatment 42·3% of the adults (n=104) were homozygous TTC/TTC, 55·8% were heterozygous TTC/TAC and only 1·9% showed the homozygous genotype TAC/TAC. The results of the molecular analysis lead to the proposal that polymorphism within codon 200 is not the only reason for the development of BZ resistance in small strongyles.

Keywords

horse strongylesCyathostominaeanthelmintic resistancebenzimidazolesbeta-tubulin genePCR based analysis
Type
Research Article
Information
Parasitology , Volume 127 , Issue 1 , July 2003 , pp. 53 - 59
Copyright
2003 Cambridge University Press

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