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Antibody dependent cell-mediated cytotoxicity of Trypanosoma cruzi: the release of tritium-labelled RNA, DNA and protein

Published online by Cambridge University Press:  06 April 2009

C. J. Sanderson ,
Marlene M. Bunn Moreno  and
Angel F. Lopez
C. J. Sanderson
Affiliation:
Instituto de Biofísica and Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal Rio de Janeiro, Cidade Universitária – ZC 32, Rio de Janeiro, RJ 20.000, Brasil
Marlene M. Bunn Moreno
Affiliation:
Instituto de Biofísica and Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal Rio de Janeiro, Cidade Universitária – ZC 32, Rio de Janeiro, RJ 20.000, Brasil
Angel F. Lopez
Affiliation:
Instituto de Biofísica and Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal Rio de Janeiro, Cidade Universitária – ZC 32, Rio de Janeiro, RJ 20.000, Brasil
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Summary

The cytotoxicity of normal rat spleen cells to antibody-coated Trypanosoma cruzi epimastigotes has been studied by assaying the release of [3H]-labelled macromolecules from the parasites. The release of thymidine (DNA) is slower than the release of uridine (RNA), suggesting that the nucleus is broken down more slowly than the cytoplasmic membrane. Less than 50% of the leucine (protein) is released when the parasites are lysed, whereas uridine (RNA) is almost totally released. In practical terms these results show that the release of incorporated radioisotope-labelled uridine can be used as a sensitive assay for cytotoxicity of T. cruzi. Cytotoxicity by normal rat spleen cells is antibody dependent and proportional to the logarithm of effector cell number. The lag phase and the rate of RNA release is not altered by centrifuging the parasites and effector cells to enhance contacts between them.

Type
Research Article
Information
Parasitology , Volume 76 , Issue 3 , June 1978 , pp. 299 - 307
Copyright
Copyright © Cambridge University Press 1978

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References

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