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B-1 cells contribute to susceptibility in experimental infection with Leishmania (Leishmania) chagasi

Published online by Cambridge University Press:  18 August 2015

WAGNER FRANCISCO KENNERLY MARCONDES GONZAGA ,
VANESSA XAVIER ,
BRUNO CAMOLESE VIVANCO ,
JOSÉ DANIEL LOPES  and
PATRICIA XANDER
WAGNER FRANCISCO KENNERLY MARCONDES GONZAGA
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil
VANESSA XAVIER
Affiliation:
Departamento de Ciências Biológicas, Universidade Federal de São Paulo – Campus Diadema, Brazil
BRUNO CAMOLESE VIVANCO
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil
JOSÉ DANIEL LOPES
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil Universidade Federal de São Paulo – Campus Diadema
PATRICIA XANDER*
Affiliation:
Departamento de Ciências Biológicas, Universidade Federal de São Paulo – Campus Diadema, Brazil
*
*Corresponding author. Departamento de Ciências Biológicas, Universidade Federal de São Paulo – Campus Diadema, Rua São Nicolau, 210, 4° andar, 04023-900, Diadema, Brasil. E-mail: patricia.xander@unifesp.com.
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Summary

The immune response to leishmaniasis is complex, and the result of infection depends on both the genetic composition of the Leishmania species and the immunity of the host. Clinical and experimental evidence suggest that the activation of B cells leads to exacerbation of visceral leishmaniasis. However, the role of B-1 cells (a subtype of B lymphocytes) in the pathogenesis of experimental visceral leishmaniasis has not yet been elucidated. In this study, we investigated the importance of B-1 cells in experimental infection with Leishmania. (L.) chagasi. Our results showed that BALB/XID mice (X-linked immunodeficient mice which are genetically deficient in B-1 cells) infected with L. (L.) chagasi for 45 days had a significant reduction in parasite load in the spleen when compared with control mice. Cytokine analysis showed that the BALB/XID mice had lower amounts of IL-10 in their sera compared with control group. In addition, the transfer of B-1 cells from wild type mice into IL-10KO animals led to an increase in susceptibility to L. (L.) chagasi infection in the IL-10KO mice, suggesting that the IL-10 produced by these cells is important in experimental infection. Our results suggest that B-1 cells may play an important role in susceptibility to L. (L.) chagasi.

Keywords

visceral leishmaniasisB-1 cellsIL-10
Type
Research Article
Information
Parasitology , Volume 142 , Issue 12 , October 2015 , pp. 1506 - 1515
Copyright
Copyright © Cambridge University Press 2015 

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