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Changes in the surface of Dipetalonema viteae (Filarioidea) during its development as shown by comparative peptide mapping

Published online by Cambridge University Press:  06 April 2009

Werner Baschong
Werner Baschong
Affiliation:
Swiss Tropical Institute, Socinstrasse 57, CH-4051 Basel, Switzerland
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Extract

The cuticle of parasitic nematodes, the main contact site with the host, plays an important role in host-parasite interaction and thus also in immunological control. We compared different surface-iodinated life-stages of the filarial worm Dipetalonema viteae (microfilariae, infective 3rd-stage larvae (L3), adult males and females) with respect to changes in their surface composition. Autoradiographs of peptide maps show that all stages present an identical set of peptide spots reflecting common surface protein(s). Spots specific for larvae L3 show that the composition of the iodinated surface differs in microfilariae and adults i.e. it changes during development. Adults show a spot typical for males or females. Identical spots are found in L3. This suggests that a surface component is also sex specific.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 2 , April 1985 , pp. 351 - 356
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

Adams, E. (1978). Invertebrate collagens. Science 202, 591–8.CrossRefGoogle ScholarPubMed
Baschong, W. & Rudin, W. (1982). Comparison of surface iodination methods by electron microscopic autoradiography applied in vitro to different life-stages of Dipetalonema viteae (Filarioidea). Parasitology 85, 559–65.Google Scholar
Baschong, W., Tanner, M., Betschart, B., Rudin, W. & Weiss, N. (1982). Dipetalonema viteae (Filarioidea): Extraction and immunogenicity of cuticular antigens from adult female worms. Journal of Experimental Parasitology 53, 262–9.CrossRefGoogle Scholar
Betschart, P. & Weiss, N. (1982). Approaches to the isolation of the cuticle of filariae Dipetalonema viteae for biochemical and immunological analysis. Proceedings of the 5th International Congress of Parasitology, Toronto, Canada, 1982. Molecular and Biochemical Parasitology (Suppl., 1982), 93.Google Scholar
Bird, A. F. (1980). The nematode cuticle and its surface. In Nematodes as Biological Models, vol. 2 (ed. Zuckermann, B. M.), pp. 213–36. New York and London: Academic Press.Google Scholar
Canlas, M. M. & Piessens, W. F. (1984). Stage-specific and common antigens of Brugia malayi identified with monoclonal antibodies. Journal of Immunology 132, 3138–41.Google Scholar
Clark, N. W. T., Phillip, M. & Parkhouse, R. M. E. (1982). Non-covalent interactions result in aggregation of surface antigens of the parasitic nematode Trichinella spiralis. Biochemical Journal 206, 27–32.Google Scholar
Cox, G. N., Kusch, M. & Edgar, R. S. (1981). Cuticle of Caenorhabditis elegans: its isolation and partial characterization. Journal of Cell Biology 90, 1–17.Google ScholarPubMed
Gass, R. F., Tanner, M. & Weiss, N. (1979). Development of Dipetalonema viteae third stage larvae (Nematode; Filarioidea) in micropore chambers implanted into jirds, hamsters, normal and immunized mice. Zeitschrift für Parasitenkunde 65, 488–96.Google Scholar
Gracy, R. W. (1977). Two dimensional thin layer methods. In Methods in Enzymology, vol. 47 (ed. Hirs, C. H. W. and Timasheff, S. N.), pp. 195–204. New York, San Francisco and London: Academic Press.Google Scholar
Lucius, R., Rauterberg, E. W. & Diesfeld, H. J. (1983). Identification of immunogenic proteins of Dipetalonema viteae (Filarioidea) by the ‘western blotting’ technique. Tropenmedizin und Parasitologie 34, 133–6.Google ScholarPubMed
Lumsden, D. L. (1975). Surface ultrastructure and cytochemistry of parasitic helminths. Experimental Parasitology 37, 267–339.Google Scholar
Mackenzie, C. D., Preston, P. M. & Ogilvie, B. M. (1978). Immunological properties of the surface of parasitic nematodes. Nature, London 276, 826–8.CrossRefGoogle ScholarPubMed
Maizels, R. M., Philipp, M. & Ogilvie, B. M. (1982). Molecules on the surface of parasitic nematodes as probes of the immune response in infection. Immunological Reviews 61, 110–36.CrossRefGoogle ScholarPubMed
Murray, L. W., Waite, J. H., Tanzer, M. L. & Hauschka, P. V. (1982). Preparation and charact-erisation of invertebrate collagens. In Methods in Enzymology, vol. 82 (ed. Cummingham, L. W. and Frederiksen, D. W.), pp. 65–96. New York, San Francisco and London: Academic Press.Google Scholar
Ogilvie, B. M., Philipp, M., Jungery, M., Maizeis, R. M., Worms, M. J. & Parkhouse, R. M. E. (1981). The surface of nematodes and the immune response of the host. In Proceedings of the 3rd Janssen Symposium, Biochemistry of Parasites and Host-Parasite Relationships (ed. Van der Bosch, M.), pp. 99–104. Amsterdam: Elsevier.Google Scholar
Ortega-Pierres, G., Chayen, A., Clark, N. W. T. & Parkhouse, R. M. E. (1984). The occurrence of antibodies to hidden and exposed determinants of surface antigens of Trichinella spiralis. Parasitology 88, 359–69.CrossRefGoogle ScholarPubMed
Parkhouse, R. M. E., Philip, M. & Ogilvie, B. M. (1981). Characterization of surface antigens of Trichinella spiralis infective larvae. Parasite Immunology 3, 339–52.CrossRefGoogle ScholarPubMed
Philipp, M., Parkhouse, R. M. E. & Ogilvie, B. M. (1980). Changing proteins on the surface of a parasitic nematode. Nature, London 287, 538–40.Google Scholar
Philipp, M. & Rumjaneck, F. D. (1984). Antigenic and dynamic properties of helminth surface structures. Molecular and Biochemical Parasitology 10, 245–68.CrossRefGoogle ScholarPubMed
Prüsse, A., Diesfeld, H. J. & Vollmer, S. (1982). Protein composition of various developmental stages of Dipetalonema viteae (Filarioidea). Journal of Helminthology 56, 251–5.CrossRefGoogle ScholarPubMed
Weiss, N. & Tanner, M. (1979). Studies on Dipetalonema viteae (Filarioidea). 3. Antibody-dependent cell mediated destruction of microfilariae in vivo. Tropenmedizin und Parasitologie 30, 73–80.Google ScholarPubMed
Weiss, N. & Tanner, M. (1981). Immunogenicity of the surface of filarial larvae (Dipetalonema viteae). Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 179–81.Google Scholar
Worms, M. J., Terry, R. J. & Terry, A. (1961). Dipetalonema witei, filarial parasite of the jird, Meriones libycus. I. Maintenance in the laboratory. Journal of Parasitology 47, 963–70.Google Scholar