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Characterization of cardiopulmonary function and cardiac muscarinic and adrenergic receptor density adaptation in C57BL/6 mice with chronic Trypanosoma cruzi infection

Published online by Cambridge University Press:  18 September 2006

N. N. ROCHA ,
S. GARCIA ,
L. E. D. GIMÉNEZ ,
C. C. Q. HERNÁNDEZ ,
J. F. V. SENRA ,
R. S. LIMA ,
F. CYRINO ,
E. BOUSKELA ,
M. B. P. SOARES  and
R. RIBEIRO DOS SANTOS
...Show all authors
N. N. ROCHA
Affiliation:
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil
S. GARCIA
Affiliation:
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil
L. E. D. GIMÉNEZ
Affiliation:
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
C. C. Q. HERNÁNDEZ
Affiliation:
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
J. F. V. SENRA
Affiliation:
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil
R. S. LIMA
Affiliation:
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil
F. CYRINO
Affiliation:
Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil
E. BOUSKELA
Affiliation:
Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil
M. B. P. SOARES
Affiliation:
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil
R. RIBEIRO DOS SANTOS
Affiliation:
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil
A. C. CAMPOS DE CARVALHO
Affiliation:
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
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Abstract

Circulating antibodies in chagasic patients interact with myocardial β adrenergic and muscarinic cholinergic receptors, triggering intracellular signals that alter cardiac function along the course of the disease. However, until now, experimental data in models of chronically infected chagasic mice linking the effects on myocardial β adrenergic and muscarinic receptors to cardiopulmonary dysfunction is lacking. Thus, we studied C57BL/6 mice 8 months after intraperitoneal injection of 100 trypomastigote forms of the Colombian strain of T. cruzi. Uninfected mice, matched in age, were used as controls. Histopathological analyses (inflammation and fibrosis) and radio-ligand binding assays for estimation of muscarinic and adrenergic receptor density were performed in myocardium tissue samples. When compared to controls, infected mice had electrical conduction disturbances, diastolic dysfunction, lower O2 consumption and anaerobic threshold. In addition, hearts of chronic chagasic mice had intense inflammation and fibrosis, and decreased β adrenergic and increased muscarinic receptor densities than normal controls. Our data suggest that chronic T. cruzi infection causes alterations in cardiac receptor density and fibrosis deposition which can be associated with cardiac conduction abnormalities, diastolic dysfunction and lower exercise capacity, associating for the first time all these functional and histopathological alterations in chagasic mice.

Keywords

Chagas' diseasecardiopulmonary functioncardiac receptorsmouse model
Type
Research Article
Information
Parasitology , Volume 133 , Issue 6 , December 2006 , pp. 729 - 737
Copyright
2006 Cambridge University Press

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