Hostname: page-component-848d4c4894-cjp7w Total loading time: 0 Render date: 2024-06-15T02:30:00.600Z Has data issue: false hasContentIssue false
  • English
  • Français

Characterization of Trypanosoma congolense serodemes in stocks isolated from cattle introduced onto a ranch in Kilifi, Kenya

Published online by Cambridge University Press:  06 April 2009

R. A. Masake ,
V. M. Nantulya ,
A. J. Musoke ,
S. K. Moloo  and
K. Nguli
R. A. Masake
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, KE
V. M. Nantulya
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, KE
A. J. Musoke
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, KE
S. K. Moloo
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, KE
K. Nguli
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, KE
Get access Rights & Permissions [Opens in a new window]

Summary

A herd of 20 cattle was introduced on a ranch in Kilifi, Coast Province of Kenya, where they were in contact with Glossina austeni for 6 months. In total, 65 trypanosome isolates were made from these animals. Examination of the isolates revealed that 61 were Trypanosoma congolense and 4 were T. theileri. Out of the 61 T. congolense isolates, 55 were successfully passaged and cloned in mice to provide trypanosome populations for further analyses. The stocks and their clones were inoculated into goats on which teneral G. morsitans centralis were later fed in order to provide metacyclics for use in serodeme analysis. Identification of serodemes was carried out by indirect immunofluorescence and neutralization using antimetacyclic hyperimmune sera prepared in mice against metacyclics of cloned trypanosome populations. So far 4 serodemes have been identified in 8 stocks and 7 clones. Each of the 9 stocks contained a mixture of at least 2 of the 4 serodemes identified. Furthermore, stocks isolated sequentially from individual animals contained the same serodemes despite repeated treatment with a curative dose (6 mg/kg body weight) of Berenil between isolations. From the latter finding, it can be inferred that the 4 serodemes were present on the ranch throughout the study period.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 2 , April 1987 , pp. 349 - 357
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Akol, G. W. O. & Murray, M. (1985). Induction of protective immunity in cattle by tsetse-transmitted cloned isolates of Trypanosoma congolense. Annals of Tropical Medicine and Parasitology 79, 617–27.CrossRefGoogle ScholarPubMed
Barbet, A. F., Davis, W. C. & McGuire, T. C. (1982). Cross-neutralization of two different trypanosome populations derived from a single organism. Nature, London 300, 453–6.CrossRefGoogle ScholarPubMed
Crowe, J. S., Barry, J. D., Luckins, A. G., Ross, C. A. & Vickerman, K. (1983). All metacyclic variable antigen types of Trypanosoma congolense identified using monoclonal antibodies. Nature, London 306, 389–91.CrossRefGoogle ScholarPubMed
Cunningham, M. P., Lumsden, W. H. R. & Webber, W. A. F. (1963). Preservation of viable trypanosomes in lymph tubes at low temperature. Experimental Parasitology 14, 280–4.CrossRefGoogle ScholarPubMed
Doyle, J. J. (1977). Antigenic variation in salivarian trypanosomes. In Immunity to Blood Parasites of Animals and Man, (ed. Miller, L. H., Pino, J. A. and McKelvey, J. L. Jr), pp. 31–63. New York and London: Plenum Press.CrossRefGoogle Scholar
Ford, J. (1971). The Role of the Trypanosomes in African Ecology. Oxford: Clarendon Press.Google Scholar
Geigy, R. & Kauffmann, M. (1973). Sleeping sickness survey in the Serengeti area (Tanzania) 1971. Examination of large mammals for trypanosomes. Acta Tropica 30, 12–23.Google ScholarPubMed
Moloo, S. K. (1981). Effects of maintaining Glossina morsitans morsitans on different hosts upon the vector's subsequent infection rates with pathogenic trypanosomes. Acta Tropica 38, 125–36.Google ScholarPubMed
Moloo, S. K. (1982). Cyclical transmission of pathogenic Trypanosoma species by gamma-irradiated sterile male Glossina morsitans morsitans. Parasitology 84, 289–96.CrossRefGoogle ScholarPubMed
Nantulya, V. M. (1986). Immunological approaches to the control of animal trypanosomiasis. Parasitology Today 2, 168–73.CrossRefGoogle Scholar
Nantulya, V. M., Doyle, J. J. & Jenni, L. (1978). Studies on Trypanosoma (Nannomonas) congolense II. Observations on the cyclical transmission of three field isolates by Glossina morsitans morsitans. Acta Tropica 35, 399–44.Google ScholarPubMed
Nantulya, V. M., Doyle, J. J. & Jenni, L. (1980). Studies on Trypanosoma (Nannomonas) congolense III. Antigenic variation in three cyclically transmitted stocks. Parasitology 80, 123–31.CrossRefGoogle ScholarPubMed
Nantulya, V. M., Musoke, A.J., Moloo, S. K. & Ngaira, J. M. (1983). Analysis of the variable antigen composition of Trypanosoma brucei brucei metacyclic trypanosomes using monoclonal antibodies. Acta Tropica 40, 19–24.Google ScholarPubMed
Nantulya, V. M., Musoke, A. J., Rurangirwa, F. R. & Moloo, S. K. (1984). Resistance of cattle to tsetse-transmitted challenge with Trypanosoma brucei or Trypanosoma congolense after spontaneous recovery from syringe-passaged infections. Infection and Immunity 43, 735–8.CrossRefGoogle ScholarPubMed
Paling, R. W., Leak, S. G. A., Katende, J., Kamunya, G. & Moloo, S. K. (1986). Epidemiology of animal trypanosomiasis on a cattle ranch in Kilifi, Kenya. Acta Tropica 43.Google Scholar
Van der Ploeg, L. H., Valerio, D., De Lange, T., Bernards, A., Borst, P. & Grosveld, F. G. (1982). An analysis of cosmid clones of nuclear DNA from Trypanosoma brucei shows that the genes for variant surface glycoproteins are clustered in the genome. Nucleic Acids Research 10, 5905–23.CrossRefGoogle ScholarPubMed
Van Meirvenne, N., Magnus, E. & Vervoort, T. (1977). Comparison of variable antigenic types produced by trypanosome strains of the subgenus Trypanozoon, Annales de la Société belge de Médicine tropicale 57, 409–23.Google ScholarPubMed
Wilson, A. J. (1969). Value of indirect fluorescent antibody test as a serological aid to diagnosis of Glossina-transmitted bovine trypanosomiasis. Tropical Animal Health and Production 1, 89–95.CrossRefGoogle Scholar
World Health Organization (1979). The African Trypanosomiasis. Technical Report Series 635, Geneva, Switzerland.Google Scholar